en
Scientific article
Open access
English

Long-Distance Transport of Thiamine (Vitamin B1) Is Concomitant with That of Polyamines

Published inPlant physiology, vol. 171, no. 1, p. 542-553
Publication date2016
Abstract

Thiamine (vitamin B1) is ubiquitous and essential for cell energy supply in all organisms as a vital metabolic cofactor, known for over a century. In plants, it is established that biosynthesis de novo is taking place predominantly in green tissues and is furthermore limited to plastids. Therefore, transport mechanisms are required to mediate the movement of this polar metabolite from source to sink tissue to activate key enzymes in cellular energy generating pathways but are currently unknown. Similar to thiamine, polyamines are an essential set of charged molecules required for diverse aspects of growth and development, the homeostasis of which necessitates long-distance transport processes that have remained elusive. Here, a yeast-based screen allowed us to identify Arabidopsis (Arabidopsis thaliana) PUT3 as a thiamine transporter. A combination of biochemical, physiological, and genetic approaches permitted us to show that PUT3 mediates phloem transport of both thiamine and polyamines. Loss of function of PUT3 demonstrated that the tissue distribution of these metabolites is altered with growth and developmental consequences. The pivotal role of PUT3 mediated thiamine and polyamine homeostasis in plants, and its importance for plant fitness is revealed through these findings.

Research group
Citation (ISO format)
MARTINIS, Jacopo et al. Long-Distance Transport of Thiamine (Vitamin B1) Is Concomitant with That of Polyamines. In: Plant physiology, 2016, vol. 171, n° 1, p. 542–553. doi: 10.1104/pp.16.00009
Main files (1)
Article (Published version)
accessLevelPublic
Identifiers
ISSN of the journal0032-0889
505views
234downloads

Technical informations

Creation11/03/2016 1:42:00 PM
First validation11/03/2016 1:42:00 PM
Update time03/15/2023 12:54:20 AM
Status update03/15/2023 12:54:19 AM
Last indexation01/16/2024 10:12:03 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack