Long-Distance Transport of Thiamine (Vitamin B1) Is Concomitant with That of Polyamines

Article Article scientifique Article Long-Distance Transport of Thiamine (Vitamin B1) Is Concomitant with That of Polyamines eng 2016 author Martinis Jacopo 808747 author Gas Pascual Elisabet 744148 author Szydlowski Nicolas 707804 author Crèvecoeur Michèle 22928 author Gisler Alexandra 686734 author Bürkle Lukas author Fitzpatrick Thérésa Bridget 684438 542-553 10.1104/pp.16.00009 27006489 0032-0889 Thiamine (vitamin B1) is ubiquitous and essential for cell energy supply in all organisms as a vital metabolic cofactor, known for over a century. In plants, it is established that biosynthesis de novo is taking place predominantly in green tissues and is furthermore limited to plastids. Therefore, transport mechanisms are required to mediate the movement of this polar metabolite from source to sink tissue to activate key enzymes in cellular energy generating pathways but are currently unknown. Similar to thiamine, polyamines are an essential set of charged molecules required for diverse aspects of growth and development, the homeostasis of which necessitates long-distance transport processes that have remained elusive. Here, a yeast-based screen allowed us to identify Arabidopsis (Arabidopsis thaliana) PUT3 as a thiamine transporter. A combination of biochemical, physiological, and genetic approaches permitted us to show that PUT3 mediates phloem transport of both thiamine and polyamines. Loss of function of PUT3 demonstrated that the tissue distribution of these metabolites is altered with growth and developmental consequences. The pivotal role of PUT3 mediated thiamine and polyamine homeostasis in plants, and its importance for plant fitness is revealed through these findings. Dewey 580 45 Département des sciences végétales 163 false 829 Groupe Fitzpatrick Plant physiology 171 1 a7f2c90c-d95c-4d6f-815c-03c829b722ea Article (Published version) Public Plant Physiol.-2016-Martinis-542-53.pdf 912634 application/pdf openAccess Faculté des sciences / Section de biologie / Département des sciences végétales

Title	Published in	Access level	OA Policy	Year	Views	Downloads

Long-Distance Transport of Thiamine (Vitamin B1) Is Concomitant with That of Polyamines	Plant physiology			2016	617	343

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Martinis, Jacopo