Activation of the Reg family genes by pancreatic-specific IGF-I gene deficiency and after streptozotocin-induced diabetes in mouse pancreas

Lu, Yarong; Ponton, Andre; Okamoto, Hiroshi; Takasawa, Shin; Herrera, Pedro Luis; Liu, J. L.

doi:10.1152/ajpendo.00596.2005

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Title		Activation of the Reg family genes by pancreatic-specific IGF-I gene deficiency and after streptozotocin-induced diabetes in mouse pancreas
Authors		Lu, Yarong Ponton, Andre Okamoto, Hiroshi Takasawa, Shin Herrera, Pedro Luis Liu, J. L.
Published in		American Journal of Physiology. Endocrinology and Metabolism. 2006, vol. 291, no. 1, p. E50-58
Abstract		We have recently reported that Pdx1-Cre-mediated whole pancreas inactivation of IGF-I gene [in pancreatic-specific IGF-I gene-deficient (PID) mice] results in increased beta-cell mass and significant protection against both type 1 and type 2 diabetes. Because the phenotype is unlikely a direct consequence of IGF-I deficiency, the present study was designed to explore possible activation of proislet factors in PID mice by using a whole genome DNA microarray. As a result, multiple members of the Reg family genes (Reg2, -3alpha, and -3beta, previously not known to promote islet cell growth) were significantly upregulated in the pancreas. This finding was subsequently confirmed by Northern blot and/or real-time PCR, which exhibited 2- to 8-fold increases in the levels of these mRNAs. Interestingly, these Reg family genes were also activated after streptozotocin-induced beta-cell damage and diabetes (wild-type T1D mice) when islet cells were undergoing regeneration. Immunohistochemistry revealed increased Reg proteins in exocrine as well as endocrine pancreas and suggested their potential role in beta-cell neogenesis in PID or T1D mice. Previously, other Reg proteins (Reg1 and islet neogenesis-associated protein) have been shown to promote islet cell replication and neogenesis. These uncharacterized Reg proteins may play a similar but more potent role, not only in normal islet cell growth in PID mice, but also in islet cell regeneration after T1D.
Keywords		Animals — Blotting, Northern — Crosses, Genetic — Diabetes Mellitus, Experimental/ genetics/metabolism/pathology — Diabetes Mellitus, Type 1/ genetics/metabolism/pathology — Female — Gene Expression Regulation — Immunohistochemistry — Insulin-Like Growth Factor I/ deficiency/genetics — Islets of Langerhans/ metabolism/pathology — Lithostathine/biosynthesis/ genetics — Male — Mice — Mice, Inbred C57BL — Mice, Inbred DBA — Mice, Transgenic — Oligonucleotide Array Sequence Analysis — RNA, Messenger/biosynthesis/genetics — Reverse Transcriptase Polymerase Chain Reaction
Identifiers		DOI: 10.1152/ajpendo.00596.2005 PMID: 16449294
Full text		Article - Limited access to UNIGE
Structures		Faculté de médecine / Section de médecine clinique / Département de médecine
Research group		Types cellulaires pancréatiques pendant l'ontogénèse (522)
Citation (ISO format)		LU, Yarong et al. Activation of the Reg family genes by pancreatic-specific IGF-I gene deficiency and after streptozotocin-induced diabetes in mouse pancreas. In: American Journal of Physiology. Endocrinology and Metabolism, 2006, vol. 291, n° 1, p. E50-58. doi: 10.1152/ajpendo.00596.2005 https://archive-ouverte.unige.ch/unige:8868