UNIGE document Scientific Article
previous document  unige:8831  next document
add to browser collection
Title

Identification and characterization of alpha 3 beta 1 integrin on primary and transformed rat islet cells

Authors
Kantengwa, S.
Baetens, D.
Sadoul, K.
Buck, C. A.
Published in Experimental Cell Research. 1997, vol. 237, no. 2, p. 394-402
Abstract Dispersed rat islet cells embedded in a matrix of collagen I are known to form aggregates in vitro reminiscent of native islets. Furthermore, it appears that islet function and survival are better maintained in vitro when cells are grown in the presence of extracellular matrix. These studies suggest an important role of cell--matrix interactions in the formation and maintenance of islet structure and function. The molecular basis of these interactions is mostly unknown. In the present study, we confirm the presence of beta 1 integrins on primary and transformed (RIN-2A line) rat islet cells. Perturbation studies in vitro show that beta 1 integrins play a role in islet cell attachment and spreading on bovine extracellular matrix and on the matrix produced by A-431 cells. The alpha 3 integrin subunit is coimmunoprecipitated with beta 1 from extracts of both primary and transformed islet cells, and immunodepletion studies suggest that alpha 3 beta 1 represents nearly half of the total beta 1 integrins expressed on primary islet cells. In situ, alpha 3 and beta 1 are expressed on the surface of all islet cell types, as shown by indirect immunocytochemistry on paraformaldehyde-fixed sections of rat pancreas. In conclusion, the study demonstrates the presence of alpha 3 beta 1 on primary and transformed rat islet cells, and an important role of beta 1 integrins in islet cell attachment and spreading in vitro.
Keywords AnimalsAntigens, CD/ chemistry/metabolismAntigens, CD29/ chemistry/metabolismBlotting, WesternCell AdhesionCell Adhesion Molecules/ chemistry/metabolismCell Transformation, Neoplastic/ pathologyExtracellular Matrix/physiologyIntegrin alpha3Integrins/ chemistry/metabolismIslets of Langerhans/ cytology/metabolism/ultrastructureMaleMolecular WeightPrecipitin TestsRatsRats, Sprague-Dawley
Identifiers
PMID: 9434635
Full text
Article - document accessible for UNIGE members only Limited access to UNIGE
Structures
Citation
(ISO format)
KANTENGWA, S. et al. Identification and characterization of alpha 3 beta 1 integrin on primary and transformed rat islet cells. In: Experimental Cell Research, 1997, vol. 237, n° 2, p. 394-402. https://archive-ouverte.unige.ch/unige:8831

172 hits

0 download

Update

Deposited on : 2010-07-12

Export document
Format :
Citation style :