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Scientific article
Open access
English

Haemocompatibility evaluation of DLC- and SIC-coated surfaces

Published inEuropean cells & materials, vol. 5, p. 17-28
Publication date2003
Abstract

Diamond-like carbon (DLC) and silicon carbide (SiC) coatings are attractive because of low friction coefficient, high hardness, chemical inertness and smooth finish, which they provide to biomedical devices. Silicon wafers (Siwaf) and silicone rubber (Sirub) plates were coated using plasmaenhanced chemical vapour deposition (PE-CVD) techniques. This article describes: 1- the characterization of modified surfaces using attenuated total reflection-Fourier transform infrared spectroscopy (ATR/FTIR) and contact angle measurements, 2- the results of three in-vitro haemocompatibility assays. Coated surfaces were compared to uncoated materials and various substrates such as polymethylmethacrylate (PMMA), polyethylene (LDPE), polydimethylsiloxane (PDMS) and medical steel (MS). Thrombin generation, blood platelet adhesion and complement convertase activity tests revealed the following classification, from the most to the least heamocompatible surface: Sirub/ DLC-Sirub/ DLC-Siwaf/ LDPE/ PDMS/ SiCSi waf/ Siwaf/ PMMA/ MS. The DLC coating surfaces delayed the clotting time, tended to inhibit the platelet and complement convertase activation, whereas SiC-coated silicon wafer can be considered as thrombogenic. This study has taken into account three events of the blood activation: coagulation, platelet activation and inflammation. The response to those events is an indicator of the in vitro haemocompatibility of the different surfaces and it allows us to select biomaterials for further in vivo blood contacting investigations.

Keywords
  • Diamond-like carbon coating
  • Plasma deposition
  • platelet adhesion
  • Thrombin generation
  • Complement convertase attachment
Funding
  • Autre - This work is supported by the COST Action 527 (Con- tract n° BBW/OFES-NBC01.0051) from the Swiss government
Citation (ISO format)
NURDIN, Nathalie et al. Haemocompatibility evaluation of DLC- and SIC-coated surfaces. In: European cells & materials, 2003, vol. 5, p. 17–28.
Main files (1)
Article (Published version)
accessLevelPublic
Identifiers
  • PID : unige:88239
ISSN of the journal1473-2262
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370downloads

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