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Plasminogen activator and mouse spermatozoa: urokinase synthesis in the male genital tract and binding of the enzyme to the sperm cell surface

Published in The Journal of Cell Biology. 1987, vol. 104, no. 5, p. 1281-1289
Abstract When ejaculated mouse spermatozoa were embedded in a plasminogen-containing insoluble protein substrate, a zone of proteolysis developed progressively, centered around the sperm head region. Lysis did not occur in absence of plasminogen or in presence of antibodies against the urokinase-type plasminogen activator (u-PA). Zymographic and immunological analyses confirmed the presence of u-PA in extracts of ejaculated mouse spermatozoa. In contrast, the u-PA activity of sperm cells obtained from testis or from vas deferens was low, although these cells were able to bind added murine u-PA. The sites of u-PA synthesis were identified by measuring u-PA activity and u-PA mRNA content in protein extracts and in total RNA preparations of various portions of the male genital tract. The highest levels of u-PA activity and of u-PA mRNA were found in vas deferens and seminal vesicles. The cells that synthesize u-PA were localized by hybridizing frozen sections of various portions of the genital tract to a u-PA cRNA probe. In all tissues examined, u-PA mRNA was predominantly located in the epithelial layer, and the strongest signal was observed over that of the vas deferens. Hence, the u-PA associated with ejaculated sperm cells is probably acquired from genital tract secretions. Sperm-bound u-PA may participate in the proteolytic events that accompany capacitation and fertilization.
Keywords AnimalsCell Membrane/metabolismEnzyme Precursors/ biosynthesisMaleMiceMice, Inbred StrainsNucleic Acid HybridizationPlasmidsPlasminogen Activators/ biosynthesis/genetics/metabolismProtein BindingSpermatozoa/ enzymologyTestis/physiologyTranscription, GeneticUrokinase-Type Plasminogen Activator/ biosynthesis/genetics/metabolism
PMID: 3106363
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Other version: http://jcb.rupress.org/content/104/5/1281.full.pdf
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HUARTE, Joachim et al. Plasminogen activator and mouse spermatozoa: urokinase synthesis in the male genital tract and binding of the enzyme to the sperm cell surface. In: The Journal of Cell Biology, 1987, vol. 104, n° 5, p. 1281-1289. doi: 10.1083/jcb.104.5.1281 https://archive-ouverte.unige.ch/unige:8816

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Deposited on : 2010-07-12

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