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Two transgenic approaches to define the cell lineages in endocrine pancreas development

Published in Molecular and cellular endocrinology. 1998, vol. 140, no. 1-2, p. 45-50
Abstract Ontogenic relationships between the different endocrine cell types of the islets of Langerhans were explored by generating transgenic mice, in which cells transcribing the glucagon, insulin, or pancreatic polypeptide genes were destroyed through the promoter-targeted expression of the diphtheria toxin A chain. In an alternate approach, to assess whether insulin cells are derived from precursors producing glucagon or PP, transgenic mice were generated bearing an insulin promoter-driven, and loxP-containing ('floxed') reporter transgene that can be irreversibly 'tagged' by recombination. They were crossed with mice expressing another transgene ('tagger') encoding Cre (cyclization recombination) recombinase in either glucagon or PP cells. The results obtained using both approaches indicate that neither glucagon nor insulin gene-expressing cells are the precursors to the other islet cells; also, they suggest that PP gene-expressing cells are necessary for the differentiation of islet insulin and somatostatin cells, through a cell lineage or a paracrine relationship.
Keywords AnimalsCell DifferentiationCell LineageDiphtheria Toxin/genetics/toxicityGene Expression RegulationGenes, Reporter/geneticsIntegrases/geneticsIslets of Langerhans/ cytology/growth & developmentMiceMice, TransgenicPancreatic Hormones/biosynthesis/geneticsPeptide Fragments/genetics/toxicityRecombination, GeneticRegulatory Sequences, Nucleic AcidStem Cells/cytologyTransgenes/ geneticsViral Proteins
PMID: 9722167
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HERRERA, Pedro Luis, ORCI, Lelio, VASSALLI, Jean-Dominique. Two transgenic approaches to define the cell lineages in endocrine pancreas development. In: Molecular and cellular endocrinology, 1998, vol. 140, n° 1-2, p. 45-50. doi: 10.1016/s0303-7207(98)00028-8 https://archive-ouverte.unige.ch/unige:8811

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Deposited on : 2010-07-12

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