Rapid Diagnosis of Infection in the Critically Ill, a Multicenter Study of Molecular Detection in Bloodstream Infections, Pneumonia, and Sterile Site Infections

Vincent, Jean-Louis; Brealey, David; Libert, Nicolas; Abidi, Nour Elhouda; O'Dwyer, Michael; Zacharowski, Kai; Mikaszewska-Sokolewicz, Malgorzata; Schrenzel, Jacques; Simon, François; Wilks, Mark; Picard-Maureau, Marcus; Chalfin, Donald B; Ecker, David J; Sampath, Rangarajan; Singer, Mervyn

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Title		Rapid Diagnosis of Infection in the Critically Ill, a Multicenter Study of Molecular Detection in Bloodstream Infections, Pneumonia, and Sterile Site Infections
Authors		Vincent, Jean-Louis Brealey, David Libert, Nicolas Abidi, Nour Elhouda O'Dwyer, Michael Zacharowski, Kai Mikaszewska-Sokolewicz, Malgorzata Schrenzel, Jacques Simon, François Wilks, Mark Picard-Maureau, Marcus Chalfin, Donald B Ecker, David J Sampath, Rangarajan Singer, Mervyn show all authors [1 - 15]
Published in		Critical Care Medicine. 2015, vol. 43, no. 11, p. 2283-2291
Abstract		Early identification of causative microorganism(s) in patients with severe infection is crucial to optimize antimicrobial use and patient survival. However, current culture-based pathogen identification is slow and unreliable such that broad-spectrum antibiotics are often used to insure coverage of all potential organisms, carrying risks of overtreatment, toxicity, and selection of multidrug-resistant bacteria. We compared the results obtained using a novel, culture-independent polymerase chain reaction/electrospray ionization-mass spectrometry technology with those obtained by standard microbiological testing and evaluated the potential clinical implications of this technique.
Keywords		Adult — Aged — Bacteremia/diagnosis/microbiology — Blood-Borne Pathogens/isolation & purification — Body Fluids/microbiology — Critical Care/methods — Critical Illness — Early Diagnosis — Europe — Female — Gram-Negative Bacteria/isolation & purification — Gram-Positive Bacteria/isolation & purification — Humans — Intensive Care Units — Male — Middle Aged — Molecular Diagnostic Techniques — Pneumonia, Bacterial/diagnosis/microbiology — Polymerase Chain Reaction/methods — Risk Assessment — Sensitivity and Specificity — Specimen Handling — Spectrometry, Mass, Electrospray Ionization/methods
Identifiers		DOI: 10.1097/CCM.0000000000001249 PMID: 26327198
Full text		Article (Published version) (718 Kb) - Free access Other version: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4603364/pdf/ccm-43-2283.pdf
Structures		Faculté de médecine / Section de médecine clinique / Département de médecine interne des spécialités
Research group		Analyse génomique et fonctionnelle du staphylocoque doré (604)
Citation (ISO format)		VINCENT, Jean-Louis et al. Rapid Diagnosis of Infection in the Critically Ill, a Multicenter Study of Molecular Detection in Bloodstream Infections, Pneumonia, and Sterile Site Infections. In: Critical Care Medicine, 2015, vol. 43, n° 11, p. 2283-2291. https://archive-ouverte.unige.ch/unige:87996