en
Scientific article
Open access
English

Rapid Diagnosis of Infection in the Critically Ill, a Multicenter Study of Molecular Detection in Bloodstream Infections, Pneumonia, and Sterile Site Infections

Published inCritical care medicine, vol. 43, no. 11, p. 2283-2291
Publication date2015
Abstract

Early identification of causative microorganism(s) in patients with severe infection is crucial to optimize antimicrobial use and patient survival. However, current culture-based pathogen identification is slow and unreliable such that broad-spectrum antibiotics are often used to insure coverage of all potential organisms, carrying risks of overtreatment, toxicity, and selection of multidrug-resistant bacteria. We compared the results obtained using a novel, culture-independent polymerase chain reaction/electrospray ionization-mass spectrometry technology with those obtained by standard microbiological testing and evaluated the potential clinical implications of this technique.

Keywords
  • Adult
  • Aged
  • Bacteremia/diagnosis/microbiology
  • Blood-Borne Pathogens/isolation & purification
  • Body Fluids/microbiology
  • Critical Care/methods
  • Critical Illness
  • Early Diagnosis
  • Europe
  • Female
  • Gram-Negative Bacteria/isolation & purification
  • Gram-Positive Bacteria/isolation & purification
  • Humans
  • Intensive Care Units
  • Male
  • Middle Aged
  • Molecular Diagnostic Techniques
  • Pneumonia, Bacterial/diagnosis/microbiology
  • Polymerase Chain Reaction/methods
  • Risk Assessment
  • Sensitivity and Specificity
  • Specimen Handling
  • Spectrometry, Mass, Electrospray Ionization/methods
Citation (ISO format)
VINCENT, Jean-Louis et al. Rapid Diagnosis of Infection in the Critically Ill, a Multicenter Study of Molecular Detection in Bloodstream Infections, Pneumonia, and Sterile Site Infections. In: Critical care medicine, 2015, vol. 43, n° 11, p. 2283–2291. doi: 10.1097/CCM.0000000000001249
Main files (1)
Article (Published version)
accessLevelPublic
Identifiers
ISSN of the journal0090-3493
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