Scientific article

The degradation of semisynthetic tritiated insulin by perfused mouse livers

Published inBiochemical journal, vol. 160, no. 2, p. 409-412
Publication date1976

Semisynthetic [3H]insulin was stored under various conditions for up to 180 days and the stability of the insulin under these conditions was assessed. A sample that had been stored for 180 days was repurified and shown to be degraded at the same rate as native insulin by perfused mouse livers, even at low physiological concentrations. After perfusion, intact insulin could be separated from degradation products, and the radioactivity associated with the insulin fraction could be used to determine the percentage degradation. The initial rate of degradation of insulin was a linear function of concentration over the range 360pM-1.9nM.

  • Animals
  • Chromatography, Gel
  • Drug Storage
  • Hormones/ metabolism
  • Insulin/isolation & purification/ metabolism
  • Liver/ metabolism
  • Mice
Citation (ISO format)
HALBAN, Philippe A. et al. The degradation of semisynthetic tritiated insulin by perfused mouse livers. In: Biochemical journal, 1976, vol. 160, n° 2, p. 409–412. doi: 10.1042/bj1600409
Updates (1)
ISSN of the journal0264-6021

Technical informations

Creation07/12/2010 11:58:10 AM
First validation07/12/2010 11:58:10 AM
Update time03/14/2023 3:51:12 PM
Status update03/14/2023 3:51:12 PM
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