UNIGE document Scientific Article
previous document  unige:87793  next document
add to browser collection
Title

WASH drives early recycling from macropinosomes and phagosomes to maintain surface phagocytic receptors

Authors
Buckley, Catherine M
Johnston, Simon A
Insall, Robert H
King, Jason S
Published in Proceedings of the National Academy of Sciences. 2016, vol. 113, no. 40, p. E5906-E5915
Abstract Macropinocytosis is an ancient mechanism that allows cells to harvest nutrients from extracellular media, which also allows immune cells to sample antigens from their surroundings. During macropinosome formation, bulk plasma membrane is internalized with all its integral proteins. It is vital for cells to salvage these proteins before degradation, but the mechanisms for sorting them are not known. Here we describe the evolutionarily conserved recruitment of the WASH (WASP and SCAR homolog) complex to both macropinosomes and phagosomes within a minute of internalization. Using Dictyostelium, we demonstrate that WASH drives protein sorting and recycling from macropinosomes and is thus essential to maintain surface receptor levels and sustain phagocytosis. WASH functionally interacts with the retromer complex at both early and late phases of macropinosome maturation, but mediates recycling via retromer-dependent and -independent pathways. WASH mutants consequently have decreased membrane levels of integrins and other surface proteins. This study reveals an important pathway enabling cells to sustain macropinocytosis without bulk degradation of plasma membrane components.
Identifiers
PMID: 27647881
Full text
Article (Published version) (2.7 MB) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Research group Groupe Soldati
Citation
(ISO format)
BUCKLEY, Catherine M et al. WASH drives early recycling from macropinosomes and phagosomes to maintain surface phagocytic receptors. In: Proceedings of the National Academy of Sciences, 2016, vol. 113, n° 40, p. E5906-E5915. https://archive-ouverte.unige.ch/unige:87793

139 hits

1 download

Update

Deposited on : 2016-09-27

Export document
Format :
Citation style :