UNIGE document Scientific Article
previous document  unige:87786  next document
add to browser collection
Title

Social amoebae trap and kill bacteria by casting DNA nets

Authors
Zhuchenko, Olga
Kuspa, Adam
Published in Nature Communications. 2016, vol. 7, p. 10938
Abstract Extracellular traps (ETs) from neutrophils are reticulated nets of DNA decorated with anti-microbial granules, and are capable of trapping and killing extracellular pathogens. Various phagocytes of mammals and invertebrates produce ETs, however, the evolutionary history of this DNA-based host defence strategy is unclear. Here we report that Sentinel (S) cells of the multicellular slug stage of the social amoeba Dictyostelium discoideum produce ETs upon stimulation with bacteria or lipopolysaccharide in a reactive oxygen species-dependent manner. The production of ETs by S cells requires a Toll/Interleukin-1 receptor domain-containing protein TirA and reactive oxygen species-generating NADPH oxidases. Disruption of these genes results in decreased clearance of bacterial infections. Our results demonstrate that D. discoideum is a powerful model organism to study the evolution and conservation of mechanisms of cell-intrinsic immunity, and suggest that the origin of DNA-based ETs as an innate immune defence predates the emergence of metazoans.
Keywords BacteriaDNA/physiologyDictyostelium/physiologyExtracellular Traps/physiologyKlebsiella pneumoniae/physiologyMicrobial ViabilityPseudomonas aeruginosa/physiologyReactive Oxygen Species
Identifiers
PMID: 26927887
Full text
Article (Published version) (1.6 MB) - public document Free access
Structures
Research group Groupe Soldati
Citation
(ISO format)
ZHANG, Xuezhi et al. Social amoebae trap and kill bacteria by casting DNA nets. In: Nature Communications, 2016, vol. 7, p. 10938. https://archive-ouverte.unige.ch/unige:87786

133 hits

36 downloads

Update

Deposited on : 2016-09-27

Export document
Format :
Citation style :