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Establishment of reptilian primary cell cultures

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Denomination Master GDE
Defense Maîtrise : Univ. Genève, 2016
Abstract Cell culture is one of the main tools that researches use to answer questions in the field of biology. It represents a simplified in vivo model of the subject, and the human body is certainly the principle topic under investigation. For this reason, cell cultures from various model organisms such as rodents are widely available, but this is not the case for cell cultures of non-classical model animals. In this work we focused on model species of reptiles, promoted by the research work in our laboratory, on which we tested different protocols for the isolation of primary cells, fibroblasts, in order to find a viable and perdurable way to establish primary cell cultures. Tests were done using different organ samples collected from the various species of snakes and lizards and by evaluating the proprieties of different culture media and enzyme digestions.
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CASTELLUCCI, Emilio. Establishment of reptilian primary cell cultures. Université de Genève. Maîtrise, 2016. https://archive-ouverte.unige.ch/unige:87473

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Deposited on : 2016-09-19

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