Establishment of reptilian primary cell cultures

Diplôme Master Establishment of reptilian primary cell cultures eng 2016 author Castellucci Emilio 829658 collaborator Tzika Athanasia 683179 director Milinkovitch Michel C. 679786 Cell culture is one of the main tools that researches use to answer questions in the field of biology. It represents a simplified in vivo model of the subject, and the human body is certainly the principle topic under investigation. For this reason, cell cultures from various model organisms such as rodents are widely available, but this is not the case for cell cultures of non-classical model animals. In this work we focused on model species of reptiles, promoted by the research work in our laboratory, on which we tested different protocols for the isolation of primary cells, fibroblasts, in order to find a viable and perdurable way to establish primary cell cultures. Tests were done using different organ samples collected from the various species of snakes and lizards and by evaluating the proprieties of different culture media and enzyme digestions. Dewey 590 Master GDE 44 Département de génétique et évolution 162 false 5b4a92d1-3a2c-48a9-ace3-57927372e876 Master thesis Restricted Establishment.pdf 17035360 application/pdf restrictedAccess Faculté des sciences / Section de biologie / Département de génétique et évolution

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Establishment of reptilian primary cell cultures				2016	159	12

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Castellucci, Emilio