UNIGE document Scientific Article
previous document  unige:8732  next document
add to browser collection
Title

FSH-stimulated PTEN activity accounts for the lack of FSH mitogenic effect in prepubertal rat Sertoli cells

Authors
Dupont, Joelle
Musnier, Astrid
Decourtye, Jeremy
Boulo, Thomas
Lecureuil, Charlotte
Guillou, Herve
Valet, Sophie
Fouchecourt, Sophie
show hidden authors show all authors [1 - 12]
Published in Molecular and Cellular Endocrinology. 2010, vol. 315, no. 1-2, p. 271-276
Abstract Follicle-stimulating hormone (FSH) controls the proliferation and differentiation of Sertoli cells of the testis. FSH binds a G protein-coupled receptor (GPCR) to stimulate downstream effectors of the phosphoinositide-3 kinase (PI3K)-dependent pathway, without enhancing PI3K activity. To clarify this paradox, we explored the activity of phosphatase and tensin homolog deleted in chromosome 10 (PTEN), the PI3K major regulator, in primary cultures of rat Sertoli cells. We show that, within minutes, FSH increases PTEN neo-synthesis, requiring the proteasomal degradation of an unidentified intermediate, as well as PTEN enzymatic activity. Importantly, introducing an antisense cDNA of PTEN into differentiating Sertoli cells restores FSH-dependent cell proliferation. In conclusion, these results provide a new mechanism of PTEN regulation, which could serve to block entry into S phase of Sertoli cells, while they are proceeding through differentiation in prepubertal animals.
Identifiers
PMID: 19778579
Full text
Article - document accessible for UNIGE members only Limited access to UNIGE
Structures
Citation
(ISO format)
DUPONT, Joelle et al. FSH-stimulated PTEN activity accounts for the lack of FSH mitogenic effect in prepubertal rat Sertoli cells. In: Molecular and Cellular Endocrinology, 2010, vol. 315, n° 1-2, p. 271-276. https://archive-ouverte.unige.ch/unige:8732

115 hits

1 download

Update

Deposited on : 2010-07-12

Export document
Format :
Citation style :