UNIGE document Scientific Article
previous document  unige:8690  next document
add to browser collection

Expression of neural cell adhesion molecule (N-CAM) in rat islets and its role in islet cell type segregation

Cirulli, V.
Baetens, D.
Rutishauser, U.
Published in Journal of Cell Science. 1994, vol. 107, no. 6, p. 1429-1436
Abstract Endocrine cell types are non-randomly distributed within pancreatic islets of Langerhans. In the rat, insulin-secreting B-cells occupy the core of the islets and are surrounded by A-, D- and PP-cells, secreting glucagon, somatostatin and pancreatic polypeptide, respectively. Furthermore, dissociated islet cells have the ability in vitro to form aggregates with the same cell-type organization as native islets (pseudoislets). These observations suggest that a differential expression of cell adhesion molecules (CAMs) might characterize B- and non-B-cells (A-, D- and PP-cells), and be in part responsible for the establishment and maintenance of islet architecture. Indirect immunofluorescence using antibodies against CAMs and islet hormones was performed on serial sections of the splenic and duodenal parts of the rat pancreas. Staining for the Ca(2+)-dependent CAM E-cadherin was detected on both exocrine and endocrine tissue and was uniform over the entire islet section, in both pancreatic regions. By contrast, staining for the Ca(2+)-independent neural CAM (N-CAM) was restricted to endocrine tissue and nerve endings. Furthermore, N-CAM staining of endocrine cells was stronger in the islet periphery, a region composed mostly of non-B-cells. Serial sections demonstrate that cells staining strongly for N-CAM in the splenic part correspond to glucagon cells and in the duodenal part to pancreatic polypeptide cells. Within pseudoislets in vitro a stronger staining for N-CAM was also observed on peripheral cells, corresponding to non-B-cells.
Keywords AnimalsCadherins/biosynthesisCell Adhesion Molecules, Neuronal/ biosynthesis/physiologyCell Aggregation/physiologyCell SeparationFluorescent Antibody TechniqueGene Expression RegulationGlucagon/analysisImmunoglobulin Fab Fragments/immunologyInsulin/analysisIslets of Langerhans/cytology/ metabolismPancreas/ cytologyRats
PMID: 7962186
Full text
(ISO format)
CIRULLI, V. et al. Expression of neural cell adhesion molecule (N-CAM) in rat islets and its role in islet cell type segregation. In: Journal of Cell Science, 1994, vol. 107, n° 6, p. 1429-1436. https://archive-ouverte.unige.ch/unige:8690

266 hits



Deposited on : 2010-07-12

Export document
Format :
Citation style :