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A new, rapid, method for preparation of dispersed pancreatic acini

Bruzzone, R.
Gjinovci, A.
Trimble, E. R.
Published in Biochemical Journal. 1985, vol. 226, no. 2, p. 621-624
Abstract A new method for the preparation of pancreatic acini is described. The method is simple and much more rapid than previously described techniques, the time required for preparation of pancreatic acini being 20 min from removal of the pancreas. Acini prepared with this method perform in a superior manner when stimulated by either caerulein or secretin. Thus this new technique would be ideal for use in binding and secretion studies.
Keywords Amylases/metabolismAnimalsCaerulein/pharmacologyCell Separation/ methodsDose-Response Relationship, DrugMalePancreas/ cytology/drug effects/enzymologyRatsRats, Inbred StrainsSecretin/pharmacology
PMID: 2581556
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BRUZZONE, R. et al. A new, rapid, method for preparation of dispersed pancreatic acini. In: Biochemical Journal, 1985, vol. 226, n° 2, p. 621-624. https://archive-ouverte.unige.ch/unige:8657

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Deposited on : 2010-07-12

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