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Scientific article
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Allosterically Regulated Phosphatase Activity from Peptide-PNA Conjugates Folded Through Hybridization

Published inAngewandte Chemie, vol. 55, no. 30, p. 8595-8598
Publication date2016
Abstract

The importance of spatial organization in short peptide catalysts is well recognized. We synthesized and screened a library of peptides flanked by peptide nucleic acids (PNAs) such that the peptide would be constrained in a hairpin loop upon hybridization. A screen for phosphatase activity led to the discovery of a catalyst with >25-fold rate acceleration over the linear peptide. We demonstrated that the hybridization-enforced folding of the peptide is necessary for activity, and designed a catalyst that is allosterically controlled using a complementary PNA sequence.

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Citation (ISO format)
MACHIDA, Takuya, DUTT, Som, WINSSINGER, Nicolas. Allosterically Regulated Phosphatase Activity from Peptide-PNA Conjugates Folded Through Hybridization. In: Angewandte Chemie, 2016, vol. 55, n° 30, p. 8595–8598. doi: 10.1002/anie.201602751
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ISSN of the journal1433-7851
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Creation07/13/2016 5:53:00 PM
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