Scientific article
Open access

Characterization and classification of matrix effects in biological samples analyses

Published inJournal of chromatography, vol. 1217, no. 25, p. 4071-4078
Publication date2010

An exhaustive classification of matrix effects occurring when a sample preparation is performed prior to liquid-chromatography coupled to mass spectrometry (LC-MS) analyses was proposed. A total of eight different situations were identified allowing the recognition of the matrix effect typology via the calculation of four recovery values. A set of 198 compounds was used to evaluate matrix effects after solid phase extraction (SPE) from plasma or urine samples prior to LC-ESI-MS analysis. Matrix effect identification was achieved for all compounds and classified through an organization chart. Only 17% of the tested compounds did not present significant matrix effects.

  • Matrix effects
  • Ion suppression
  • Plasma
  • Urine
  • Sample preparation
  • SPE
  • MS
Citation (ISO format)
MARCHI, Ivano et al. Characterization and classification of matrix effects in biological samples analyses. In: Journal of chromatography, 2010, vol. 1217, n° 25, p. 4071–4078. doi: 10.1016/j.chroma.2009.08.061
Main files (1)
Article (Accepted version)
ISSN of the journal0021-9673

Technical informations

Creation06/29/2010 4:51:00 PM
First validation06/29/2010 4:51:00 PM
Update time03/14/2023 3:49:34 PM
Status update03/14/2023 3:49:34 PM
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