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Determination of potassium, sodium, calcium and magnesium in total parenteral nutrition formulations by capillary electrophoresis with contactless conductivity detection
|Published in||Journal of pharmaceutical and biomedical analysis. 2010, vol. 53, no. 2, p. 130-6|
|Abstract||A simple method based on capillary electrophoresis with a capacitively coupled contactless conductivity detector (CE-C(4)D) was developed for the determination of potassium, sodium, calcium and magnesium in parenteral nutrition formulations. A hydro-organic mixture, consisting of 100 mM Tris-acetate buffer at pH 4.5 and acetonitrile (80:20, v/v), was selected as the background electrolyte. The applied voltage was 30 kV, and sample injection was performed in hydrodynamic mode. All analyses were carried out in a fused silica capillary with an internal diameter of 50 microm and a total length of 64.5 cm. Under these conditions, complete separation between all cations was achieved in less than 4 min. The CE-C(4)D method was validated, and trueness values between 98.6% and 101.8% were obtained with repeatability and intermediate precision values of 0.4-1.3% and 0.8-1.8%, respectively. Therefore, this method was found to be appropriate for controlling potassium, sodium, calcium and magnesium in parenteral nutrition formulations and successfully applied in daily quality control at the Geneva University Hospitals.|
|Keywords||Capacitively coupled contactless conductivity detection — Capillary electrophoresis — Inorganic cations — Total parenteral nutrition — Validation|
|NUSSBAUMER, Susanne et al. Determination of potassium, sodium, calcium and magnesium in total parenteral nutrition formulations by capillary electrophoresis with contactless conductivity detection. In: Journal of pharmaceutical and biomedical analysis, 2010, vol. 53, n° 2, p. 130-6. doi: 10.1016/j.jpba.2010.01.042 https://archive-ouverte.unige.ch/unige:8500|