UNIGE document Scientific Article
previous document  unige:84808  next document
add to browser collection

Transgenic analysis of a potential Hoxd-11 limb regulatory element present in tetrapods and fish

Published in Developmental biology. 1996, vol. 180, no. 2, p. 543-553
Abstract Genes of the HoxD complex related to the Drosophila Abd-B gene are involved in the morphogenesis of vertebrate paired appendages. Hoxd-11, for instance, is necessary in combination with other Hox genes for the proper development of different parts of the tetrapod limbs. Sequence comparisons between the mouse, chicken, and zebrafish Hoxd-11 loci have revealed the conservation of several blocks of DNA sequence which may be of importance for the regulation of Hoxd-11 expression. We have used transgenic mice to show that one of these conserved elements specifically drives expression in a proximal-posterior part of developing forelimbs. Production of mice transgenic for a full fish Hoxd-11 construct as well as for mouse-fish Hoxd-11 chimeric constructs shows that the fish counterpart of this sequence is able to elicit expression in mouse forelimbs as well, though in a slightly different domain. However, this fish element requires the presence of the mouse promoter and does not work in its own context. These results are discussed in light of both the control of Hoxd gene expression during limb development and the use of a comparative interspecies approach to understand the regulation of genes involved in vertebrate development.
Keywords AnimalsBase SequenceChickensCloningMolecularDNA PrimersDrosophilaForelimb/growth & developmentGene Expression RegulationDevelopmentalGenesHomeoboxHomeodomain Proteins/biosynthesis/geneticsLimb Buds/physiologyMiceMiceTransgenicMolecular Sequence DataMorphogenesisPolymerase Chain ReactionPromoter RegionsGeneticRecombinant Fusion Proteins/biosynthesisRegulatory SequencesNucleic AcidRestriction MappingSequence HomologyNucleic AcidTranscription Factors/biosynthesis/geneticsVertebratesZebrafishZebrafish Proteins
PMID: 8954726
Full text
Article (Published version) (25.1 MB) - document accessible for UNIGE members only Limited access to UNIGE
(ISO format)
BECKERS, Johannes, GERARD, Matthieu, DUBOULE, Denis. Transgenic analysis of a potential Hoxd-11 limb regulatory element present in tetrapods and fish. In: Developmental biology, 1996, vol. 180, n° 2, p. 543-553. doi: 10.1006/dbio.1996.0327 https://archive-ouverte.unige.ch/unige:84808

395 hits

1 download


Deposited on : 2016-06-28

Export document
Format :
Citation style :