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Membrane interaction of TNF is not sufficient to trigger increase in membrane conductance in mammalian cells

Fransen, Lucie
De Baetselier, Patrick
Published in FEBS Letters. 1999, vol. 460, no. 1, p. 107-11
Abstract Tumor necrosis factor TNF can trigger increases in membrane conductance of mammalian cells in a receptor-independent manner via its lectin-like domain. A lectin-deficient TNF mutant, lacking this activity, was able to bind to artificial liposomes in a pH-dependent manner, but not to insert into the bilayer, just like wild type TNF. A peptide mimicking the lectin-like domain, which can still trigger increases in membrane currents in cells, failed to interact with liposomes. Thus, the capacity of TNF to trigger increases in membrane conductance in mammalian cells does not correlate with its ability to interact with membranes, suggesting that the cytokine does not form channels itself, but rather interacts with endogenous ion channels or with plasma membrane proteins that are coupled to ion channels.
Keywords Amino Acid SequenceAnimalsCell Membrane/metabolismChlorides/metabolismCircular DichroismEscherichia coliHydrogen-Ion ConcentrationIon Channels/metabolismLiposomes/metabolismMiceModelsMolecularMolecular Sequence DataMutationPeptide Fragments/metabolismProtein BindingProtein DenaturationProtein FoldingProtein StructureSecondaryRecombinant ProteinsTumor Necrosis Factor-alpha/chemistry/genetics Tumor necrosis factorChannelMembraneUnfolding
PMID: 10571070
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VAN DER GOOT GRUNBERG, Françoise G. et al. Membrane interaction of TNF is not sufficient to trigger increase in membrane conductance in mammalian cells. In: FEBS letters, 1999, vol. 460, n° 1, p. 107-11. doi: 10.1016/s0014-5793(99)01294-6 https://archive-ouverte.unige.ch/unige:84749

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Deposited on : 2016-06-27

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