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Title

Membrane interaction of TNF is not sufficient to trigger increase in membrane conductance in mammalian cells
Authors
Van Der Goot Grunberg, Françoise G.
Pugin, Jérôme
Hribar, Marusa
Fransen, Lucie
Dunant, Yves
De Baetselier, Patrick
Bloc, Alain André Leon
Lucas, Rudolf
Published in FEBS Letters. 1999, vol. 460, no. 1, p. 107-11
Abstract Tumor necrosis factor TNF can trigger increases in membrane conductance of mammalian cells in a receptor-independent manner via its lectin-like domain. A lectin-deficient TNF mutant, lacking this activity, was able to bind to artificial liposomes in a pH-dependent manner, but not to insert into the bilayer, just like wild type TNF. A peptide mimicking the lectin-like domain, which can still trigger increases in membrane currents in cells, failed to interact with liposomes. Thus, the capacity of TNF to trigger increases in membrane conductance in mammalian cells does not correlate with its ability to interact with membranes, suggesting that the cytokine does not form channels itself, but rather interacts with endogenous ion channels or with plasma membrane proteins that are coupled to ion channels.
Keywords Amino Acid Sequence — Animals — Cell Membrane/metabolism — Chlorides/metabolism — Circular Dichroism — Escherichia coli — Hydrogen-Ion Concentration — Ion Channels/metabolism — Liposomes/metabolism — Mice — Models — Molecular — Molecular Sequence Data — Mutation — Peptide Fragments/metabolism — Protein Binding — Protein Denaturation — Protein Folding — Protein Structure — Secondary — Recombinant Proteins — Tumor Necrosis Factor-alpha/chemistry/genetics Tumor necrosis factor — Channel — Membrane — Unfolding
Identifiers
PMID: 10571070
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Article (Published version) (178 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Faculté de médecine / Section de médecine clinique / Département d'anesthésiologie, pharmacologie et soins intensifs
Faculté des sciences / Section des sciences pharmaceutiques
Faculté des sciences / Section de chimie et biochimie
Citation
(ISO format) 		VAN DER GOOT GRUNBERG, Françoise G. et al. Membrane interaction of TNF is not sufficient to trigger increase in membrane conductance in mammalian cells. In: FEBS Letters, 1999, vol. 460, n° 1, p. 107-11. https://archive-ouverte.unige.ch/unige:84749

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Deposited on : 2016-06-27

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