Micellar electrokinetic capillary chromatography for selected tropane alkaloid analysis in plant extract

Cherkaoui, Samir; Mateus, Lidia; Christen, Philippe; Veuthey, Jean-Luc

doi:10.1007/BF02490872

Scientific article

English

Micellar electrokinetic capillary chromatography for selected tropane alkaloid analysis in plant extract

Contributors

Cherkaoui, Samir; Mateus, Lidia; Christen, Philippe; Veuthey, Jean-Luc

Published in

Chromatographia, vol. 46, no. 7-8, p. 351-357

Publication date

1997

Abstract

Micellar electrokinetic capillary chromatography was applied to the simultaneous analysis of six tropane alkaloids, including hyoscyamine and scopolamine. Successful results were obtained using a 30 mM boratephosphate buffer at basic pH (8.5) in the presence of 50 mM sodium dodecyl sulfate. The operating conditions, such as buffer concentration and pH, micelle concentration and organic modifier type and percentage were also discussed on the basis of the results given with a tropane alkaloid mixture. Addition of organic modifiers showed an improvement in separation efficiency and resolution. Moreover, hyoscyamine and littorine, two positional isomers, were only resolved by the addition of organic solvents such as methanol or acetonitrile. The optimized conditions were finally applied to the analysis of tropane alkaloids found in genetically transformed root cultures ofDatura candida x D. aurea.

Keywords

Micellar electrokinetic chromatography
Tropane alkaloids
Hyoscyamine and scopolamine
Plant extract

UNIGE affiliation entities

Faculté des sciences / Section des sciences pharmaceutiques

Citation (ISO format)

CHERKAOUI, Samir et al. Micellar electrokinetic capillary chromatography for selected tropane alkaloid analysis in plant extract. In: Chromatographia, 1997, vol. 46, n° 7-8, p. 351–357. doi: 10.1007/BF02490872

Article (Published version)

Identifiers

PID : unige:8472
DOI : 10.1007/BF02490872

Additional URL for this publication

http://www.springerlink.com/index/10.1007/BF02490872

Journal ISSN

0009-5893

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28/06/2010 11:07:00

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28/06/2010 11:07:00

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26/03/2026 10:48:16

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26/03/2026 10:48:16

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26/03/2026 10:55:02

Micellar electrokinetic capillary chromatography for selected tropane alkaloid analysis in plant extract

Contributors

Published in

Publication date

Abstract

Keywords

UNIGE affiliation entities

Citation (ISO format)

Main files (1)

Identifiers

Additional URL for this publication

Journal ISSN

Technical informations

Creation

First validation

Update

Status update

Last indexation