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Scientific article
English

Nonaqueous capillary electrophoresis for the analysis of selected tropane alkaloids in a plant extract

Contributors

Cherkaoui, Samir; Mateus, Lidia; Christen, Philippe; Veuthey, Jean-Luc

Published in

Chromatographia, vol. 49, no. 1-2, p. 54-60

Publication date

1999

Abstract

The potential of nonaqueous capillary electrophoresis has been investigated for the separation of structurally similar tropane alkaloids. The effects of the organic solvent and of electrolyte composition on separation selectivity, migration times, and efficiency are described. The addition of trifluoroacetic acid to the separation buffer was found beneficial for manipulation of the order of migration of the two positional isomers littorine and hyoscyamine. Replicate injections under nonaqueous conditions gave migration time and peak area data of excellent precision. The application of the optimized conditions to the analysis of hyoscyamine and scopolamine in genetically transformed root cultures ofDatura candida x D. aurea is presented.

Keywords

  • Capillary electrophoresis
  • Micellar electrokinetic capillary chromatography
  • Nonaqueous media
  • Tropane alkaloids
  • Hyoscyamine and scopolamine

UNIGE affiliation entities

Citation (ISO format)

CHERKAOUI, Samir et al. Nonaqueous capillary electrophoresis for the analysis of selected tropane alkaloids in a plant extract. In: Chromatographia, 1999, vol. 49, n° 1-2, p. 54–60. doi: 10.1007/BF02467187

Main files (1)

Article (Published version)

Identifiers

Additional URL for this publication

http://www.springerlink.com/index/10.1007/BF02467187

Journal ISSN

0009-5893
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Technical informations

Creation

28/06/2010 11:15:00

First validation

28/06/2010 11:15:00

Update

26/03/2026 10:48:01

Status update

26/03/2026 10:48:01

Last indexation

26/03/2026 10:54:45
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