Supercritical fluid extraction and chromatography of artemisinin and artemisinic acid. An improved method for the analysis of Artemisia annua samples

Kohler, Marcel; Haerdi, Werner; Christen, Philippe; Veuthey, Jean-Luc

doi:10.1002/(SICI)1099-1565(199709/10)8:5<223::AID-PCA370>3.0.CO;2-A

Scientific article

English

Supercritical fluid extraction and chromatography of artemisinin and artemisinic acid. An improved method for the analysis of Artemisia annua samples

Contributors

Kohler, Marcel; Haerdi, Werner; Christen, Philippe; Veuthey, Jean-Luc

Published in

Phytochemical analysis, vol. 8, no. 5, p. 223-227

Publication date

1997

Abstract

Artemisinin and artemisinic acid were extracted from aerial parts of Artemisia annua by supercritical fluid extraction (SFE) with carbon dioxide modified with 3% (v/v) methanol. The quantitative determination of both compounds was carried out by supercritical fluid chromatography (SFC) coupled with an evaporative light scattering detector (SFC-ELSD). Results obtained by SFE-SFC-ELSD show that this method allows mild extraction conditions and quantitative determination without further purification of the plant extract. In all cases, artemisinin and artemisinic acid were extracted in a higher yield with supercritical carbon dioxide than with liquid-solid extraction. © 1997 John Wiley & Sons, Ltd.

Keywords

Supercritical fluid extraction
Supercritical fluid chromatography
Evaporative light scattering detector
Artemisinin
Artemisinic acid
Artemisia annua

UNIGE affiliation entities

Faculté des sciences / Section des sciences pharmaceutiques

Citation (ISO format)

KOHLER, Marcel et al. Supercritical fluid extraction and chromatography of artemisinin and artemisinic acid. An improved method for the analysis of Artemisia annua samples. In: Phytochemical analysis, 1997, vol. 8, n° 5, p. 223–227. doi: 10.1002/(SICI)1099-1565(199709/10)8:5<223::AID-PCA370>3.0.CO;2-A

Article (Published version)

Identifiers

PID : unige:8439
DOI : 10.1002/(SICI)1099-1565(199709/10)8:5<223::AID-PCA370>3.0.CO;2-A

Journal ISSN

0958-0344

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Supercritical fluid extraction and chromatography of artemisinin and artemisinic acid. An improved method for the analysis of Artemisia annua samples

Contributors

Published in

Publication date

Abstract

Keywords

UNIGE affiliation entities

Citation (ISO format)

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Technical informations

Creation

First validation

Update

Status update

Last indexation