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Title

Development of a capillary zone electrophoresis method for caseinoglycomacropeptide determination
Authors
Cherkaoui, Samir
Doumenc, N.
Tachon, P.
Neeser, J. R.
Veuthey, Jean-Luc
Published in Journal of chromatography. 1997, vol. 790, no. 1-2, p. 195-205
Abstract Caseinoglycomacropeptide (CGMP) is a polypeptide of 64 amino acid residues, derived from the C-terminal part of bovine kappa-casein. A sensitive and selective capillary zone electrophoresis method has been developed and validated for the analysis and quantitation of CGMP. Separation is carried out at 30 kV, using an uncoated fused-silica capillary and 20 mM sodium citrate buffer at acidic pH 3.5. The described method allows the separation of various CGMP subcomponents. The validation data proves that the method has the requisite selectivity, sensitivity, reproducibility and linearity for CGMP assay and for quality control during CGMP manufacturing (batch-to-batch reproducibility).
Keywords Animals — Calibration — Caseins/analysis — Cattle — Electrophoresis, Capillary — Glycopeptides/analysis — Hydrogen-Ion Concentration — Indicators and Reagents — Quality Control
Identifiers
PMID: 9435120
Full text
Article (Published version) (186 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Faculté des sciences / Section des sciences pharmaceutiques
Citation
(ISO format) 		CHERKAOUI, Samir et al. Development of a capillary zone electrophoresis method for caseinoglycomacropeptide determination. In: Journal of chromatography, 1997, vol. 790, n° 1-2, p. 195-205. doi: 10.1016/S0021-9673(97)00756-5 https://archive-ouverte.unige.ch/unige:8437

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Deposited on : 2010-06-24

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