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Scientific article
English

Development of a capillary zone electrophoresis method for caseinoglycomacropeptide determination

Contributors

Cherkaoui, Samir; Doumenc, N.; Tachon, P.; Neeser, J. R.; Veuthey, Jean-Luc

Published in

Journal of chromatography, vol. 790, no. 1-2, p. 195-205

Publication date

1997

Abstract

Caseinoglycomacropeptide (CGMP) is a polypeptide of 64 amino acid residues, derived from the C-terminal part of bovine kappa-casein. A sensitive and selective capillary zone electrophoresis method has been developed and validated for the analysis and quantitation of CGMP. Separation is carried out at 30 kV, using an uncoated fused-silica capillary and 20 mM sodium citrate buffer at acidic pH 3.5. The described method allows the separation of various CGMP subcomponents. The validation data proves that the method has the requisite selectivity, sensitivity, reproducibility and linearity for CGMP assay and for quality control during CGMP manufacturing (batch-to-batch reproducibility).

Keywords

  • Animals
  • Calibration
  • Caseins/analysis
  • Cattle
  • Electrophoresis, Capillary
  • Glycopeptides/analysis
  • Hydrogen-Ion Concentration
  • Indicators and Reagents
  • Quality Control

UNIGE affiliation entities

Citation (ISO format)

CHERKAOUI, Samir et al. Development of a capillary zone electrophoresis method for caseinoglycomacropeptide determination. In: Journal of chromatography, 1997, vol. 790, n° 1-2, p. 195–205. doi: 10.1016/S0021-9673(97)00756-5

Main files (1)

Article (Published version)

Identifiers

Journal ISSN

0021-9673
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Technical informations

Creation

22/06/2010 13:15:00

First validation

22/06/2010 13:15:00

Update

14/03/2023 15:49:20

Status update

14/03/2023 15:49:20

Last indexation

29/10/2024 15:40:45
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