Scientific article

Development of a capillary zone electrophoresis method for caseinoglycomacropeptide determination

Published inJournal of chromatography, vol. 790, no. 1-2, p. 195-205
Publication date1997

Caseinoglycomacropeptide (CGMP) is a polypeptide of 64 amino acid residues, derived from the C-terminal part of bovine kappa-casein. A sensitive and selective capillary zone electrophoresis method has been developed and validated for the analysis and quantitation of CGMP. Separation is carried out at 30 kV, using an uncoated fused-silica capillary and 20 mM sodium citrate buffer at acidic pH 3.5. The described method allows the separation of various CGMP subcomponents. The validation data proves that the method has the requisite selectivity, sensitivity, reproducibility and linearity for CGMP assay and for quality control during CGMP manufacturing (batch-to-batch reproducibility).

  • Animals
  • Calibration
  • Caseins/analysis
  • Cattle
  • Electrophoresis, Capillary
  • Glycopeptides/analysis
  • Hydrogen-Ion Concentration
  • Indicators and Reagents
  • Quality Control
Citation (ISO format)
CHERKAOUI, Samir et al. Development of a capillary zone electrophoresis method for caseinoglycomacropeptide determination. In: Journal of chromatography, 1997, vol. 790, n° 1-2, p. 195–205. doi: 10.1016/S0021-9673(97)00756-5
Main files (1)
Article (Published version)
ISSN of the journal0021-9673

Technical informations

Creation06/22/2010 1:15:00 PM
First validation06/22/2010 1:15:00 PM
Update time03/14/2023 3:49:20 PM
Status update03/14/2023 3:49:20 PM
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