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Scientific article
English

Stereoselective determination of methadone in serum by HPLC following solid-phase extraction on disk

Published inJournal of pharmaceutical and biomedical analysis, vol. 14, no. 8-10, p. 1271-1279
Publication date1996
Abstract

A solid-phase extraction (SPE) technique for methadone has been developed using a mixed-mode solid-phase extraction disk which contains both hydrophobic and cation-exchange functional groups. The SPE technique was used to isolate the drug from the biological matrix and to prepare a cleaner sample prior to stereoselective analysis by HPLC on a silica column with covalently bound alpha 1-acid glycoprotein (Chiral-AGP) followed by ultraviolet detection at 205 mm. The within-run precision was less than 5% for the complete method over the therapeutic range. The quantification limit was 25 ng ml-1. The between-run precision was less than 15% at the quantification limit. The between-run precision at other concentrations was less than 8.5% with an accuracy of more than 95%. The mean recovery for R-methadone was 78.5% and the mean recovery for S-methadone was 73.4%. The complete procedure has been validated. This method was successfully used for the analysis of 15 clinical cases.

Keywords
  • Analgesics, Opioid/blood
  • Chromatography, High Pressure Liquid/methods
  • Heroin Dependence/blood
  • Humans
  • Hydrogen-Ion Concentration
  • Methadone/blood
  • Orosomucoid/chemistry
  • Protein Binding
  • Reproducibility of Results
  • Spectrophotometry, Ultraviolet
  • Stereoisomerism
Citation (ISO format)
RUDAZ, Serge, VEUTHEY, Jean-Luc. Stereoselective determination of methadone in serum by HPLC following solid-phase extraction on disk. In: Journal of pharmaceutical and biomedical analysis, 1996, vol. 14, n° 8-10, p. 1271–1279. doi: 10.1016/0731-7085(95)01660-0
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Article (Published version)
accessLevelRestricted
Identifiers
ISSN of the journal0731-7085
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