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The lectin-like domain of tumor necrosis factor-α increases membrane conductance in microvascular endothelial cells and peritoneal macrophages

Authors
Fransen, Lucie
De Baetselier, Patrick
Grau, Georges E.
Bluethmann, Horst
Matthay, Michael A.
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Published in European Journal of Immunology. 1999, vol. 29, no. 10, p. 3105-3111
Abstract Herein, we show that TNF exerts a pH-dependent increase in membrane conductance in primary lung microvascular endothelial cells and peritoneal macrophages. This effect was TNF receptor-independent, since it also occurred in cells isolated from mice deficient in both types of TNF receptors. A TNF mutant in which the three amino acids critical for the lectin-like activity were replaced by an alanine did not show any significant effect on membrane conductance. Moreover, a synthetic 17-amino acid peptide of TNF, which was previously shown to exert lectin-like activity, also increased the ion permeability in these cells. The amiloride sensitivity of the observed activity suggests a binding of TNF to an endogenousion channel rather than channel formation by TNF itself. This may have important implications in mechanisms of TNF-mediated vascular pathology.
Keywords TNFEndotheliumConductance
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HRIBAR, Marusa et al. The lectin-like domain of tumor necrosis factor-α increases membrane conductance in microvascular endothelial cells and peritoneal macrophages. In: European Journal of Immunology, 1999, vol. 29, n° 10, p. 3105-3111. https://archive-ouverte.unige.ch/unige:84299

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Deposited on : 2016-06-08

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