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Scientific article
English

Comparison of post-column fluorescence derivatization and evaporative light-scattering detection to analyse saccharides selectively by LC

Published inChromatographia, vol. 34, no. 11-12, p. 651-654
Publication date1992
Abstract

The use of post-column fluorescence derivatization and evaporative light-scattering detection to analyse saccharides selectively by LC was investigated. In order to compare the performances of these two detection systems, factors such as reproducibility, detection limits and linear calibrations were determined. Despite the derivatization reaction step, post-column derivatization is superior from the standpoint of sensitivity. The detection of saccharides in beverages is also described.

Keywords
  • Column liquid chromatography
  • Post-column derivatization
  • Light-scattering detection
  • Carbohydrates
Citation (ISO format)
COQUET, Agnés, VEUTHEY, Jean-Luc, HAERDI, Werner. Comparison of post-column fluorescence derivatization and evaporative light-scattering detection to analyse saccharides selectively by LC. In: Chromatographia, 1992, vol. 34, n° 11-12, p. 651–654. doi: 10.1007/BF02269879
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Article (Published version)
accessLevelRestricted
Identifiers
ISSN of the journal1612-1112
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