UNIGE document Scientific Article
previous document  unige:83647  next document
add to browser collection

Interspecies exchange of a Hoxd enhancer in vivo induces premature transcription and anterior shift of the sacrum

Published in Developmental biology. 1997, vol. 190, no. 1, p. 32-40
Abstract The precise activation, in space and time, of vertebrate Hox genes is an essential requirement for normal morphogenesis. In order to assess for the functional potential of evolutionary conserved Hox regulatory sequences, a phylogenetically conserved bipartite regulatory element necessary for proper spatial and temporal activation of the Hoxd-11 gene was replaced by its fish counterpart in the HoxD complex of mice, using an ES cell-based targeted exchange. Fetuses carrying this replacement activated Hoxd-11 transcription prematurely, which led to a rostral shift of its expression boundary and a consequent anterior transposition of the sacrum. These results demonstrate the high phylogenetic conservation of regulatory mechanisms acting over vertebrate Hox complexes and suggest that minor time difference (heterochronies) in Hox gene activation may have contributed to important morphological variations in the course of evolution.
Keywords AnimalsBase SequenceCloningMolecularConserved SequenceDNARecombinantEmbryonic and Fetal DevelopmentEnhancer ElementsGenetic/physiologyGenesHomeobox/geneticsHomeodomain Proteins/geneticsMiceMiceTransgenicMolecular Sequence DataRNAMessenger/analysisSacrum/embryologySequence AlignmentSequence AnalysisDNASpecies SpecificityTranscription Factors/geneticsTranscriptional Activation/geneticsZebrafish/geneticsZebrafish Proteins
PMID: 9331329
Full text
Article (Published version) (1.2 MB) - document accessible for UNIGE members only Limited access to UNIGE
(ISO format)
GERARD, Matthieu, ZAKANY, Jozsef, DUBOULE, Denis. Interspecies exchange of a Hoxd enhancer in vivo induces premature transcription and anterior shift of the sacrum. In: Developmental biology, 1997, vol. 190, n° 1, p. 32-40. doi: 10.1006/dbio.1997.8679 https://archive-ouverte.unige.ch/unige:83647

395 hits

0 download


Deposited on : 2016-05-10

Export document
Format :
Citation style :