UNIGE document Scientific Article
previous document  unige:83156  next document
add to browser collection
Title

Genetic analysis of a conserved sequence in the HoxD complex: regulatory redundancy or limitations of the transgenic approach?

Authors
Beckers, Johannes
Published in Developmental Dynamics. 1998, vol. 213, no. 1, p. 1-11
Abstract Extensive sequencing in the HoxD complex of several vertebrate species has revealed a set of conserved DNA sequences interspersed between neighboring Hox genes. Their high degree of conservation strongly suggested that they are used for regulatory purposes, a hypothesis that was largely confirmed by using "classical transgenesis" or in vivo mutagenesis through the embryonic stem (ES) cell technology. Here, we show that this is not always the case. We report that the deletion of a conserved regulatory sequence located in the HoxD complex gives different results, depending on the transgenic approach that was used. In "conventional" transgenesis, this sequence was necessary for proper expression in a subdomain of the developing limb. However, a deletion of this sequence in complexo did not confirm this effect, thereby creating an important discrepancy between the classical transgenic and the ES cell-based, targeted mutagenesis. This unexpected observation may show the limitations of the former technology. Alternatively, it could illustrate a redundancy in regulatory circuits and, thus, justify the combination of parallel strategies.
Keywords AllelesAnimalsConserved SequenceGene Expression Regulation, DevelopmentalGene TargetingHomeodomain Proteins/geneticsMiceMice, Inbred C57BLMice, TransgenicMorphogenesisPhenotypeTranscription Factors/geneticsUrogenital System/embryology
Identifiers
PMID: 9733096
Full text
Article (Published version) (918 Kb) - public document Free access
Structures
Citation
(ISO format)
BECKERS, Johannes, DUBOULE, Denis. Genetic analysis of a conserved sequence in the HoxD complex: regulatory redundancy or limitations of the transgenic approach?. In: Developmental Dynamics, 1998, vol. 213, n° 1, p. 1-11. https://archive-ouverte.unige.ch/unige:83156

145 hits

49 downloads

Update

Deposited on : 2016-04-18

Export document
Format :
Citation style :