Optimized Generation of Functional Neutrophils and Macrophages from Patient-Specific Induced Pluripotent Stem Cells: Ex Vivo Models of X(0)-Linked, AR22(0)- and AR47(0)- Chronic Granulomatous Diseases

Brault, Julien; Goutagny, Erwan; Telugu, Narasimha; Shao, Kaifeng; Baquie, Mathurin; Satre, Véronique; Coutton, Charles; Grunwald, Didier; Brion, Jean-Paul; Barlogis, Vincent; Stephan, Jean-Louis; Plantaz, Dominique; Hescheler, Jürgen; Krause, Karl-Heinz; Sarić, Tomo; Stasia, Marie José

doi:10.1089/biores.2014.0045

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Title		Optimized Generation of Functional Neutrophils and Macrophages from Patient-Specific Induced Pluripotent Stem Cells: Ex Vivo Models of X(0)-Linked, AR22(0)- and AR47(0)- Chronic Granulomatous Diseases
Authors		Brault, Julien Goutagny, Erwan Telugu, Narasimha Shao, Kaifeng Baquie, Mathurin Satre, Véronique Coutton, Charles Grunwald, Didier Brion, Jean-Paul Barlogis, Vincent Stephan, Jean-Louis Plantaz, Dominique Hescheler, Jürgen Krause, Karl-Heinz Sarić, Tomo Stasia, Marie José show all authors [1 - 16]
Published in		BioResearch Open Access. 2014, vol. 3, no. 6, p. 311-326
Abstract		Chronic granulomatous disease (CGD) is an inherited orphan disorder caused by mutations in one of the five genes encoding reduced nicotinamide-adenine-dinucleotide-phosphate oxidase subunits, which subsequently lead to impairment in the production of microbicidal reactive oxygen species (ROS). In order to offer several cell line models of CGD and therefore support research on pathophysiology and new therapeutic approaches, we optimized protocols to differentiate induced pluripotent stem cells (iPSCs) from wild-type, X(0)-, AR22(0)- and AR47(0)-CGD patient's fibroblasts into neutrophils and into macrophages. Aberrant genetic clones were discarded after chromosome karyotyping and array-comparative genomic hybridization analysis. All remaining iPSC lines showed human embryonic stem cell-like morphology, expressed all tested pluripotency markers and formed embryoid bodies that contained cells originating from all three primary germ layers. Furthermore, each CGD patient-specific iPSC line retained the gp91 (phox) , p47 (phox) , and p22 (phox) mutations found in the corresponding patient's neutrophils. The average production of CD34(+) progenitors was of 1.5×10(6) cells after 10 days of differentiation of 10×10(6) iPSCs. They were terminally differentiated into about 3×10(5) neutrophils or into 3×10(7) macrophages. Based on morphological, phenotypical, and functional criteria both phagocyte types were mature and indistinguishable from the native human neutrophils and macrophages. However, neutrophils and macrophages derived from X(0)-, AR22(0)-, and AR47(0)-CGD patient-specific iPSC lines lacked ROS production and the corresponding mutated proteins. To simplify the phagocytes' production upon request, progenitors can be cryopreserved. In conclusion, we describe a reproducible, simple, and efficient way to generate neutrophils and macrophages from iPSCs and provide a new cellular model for the AR22(0)-CGD genetic form that has not been described before.
Identifiers		DOI: 10.1089/biores.2014.0045 PMID: 25469316
Full text		Article (Published version) (1.3 MB) - Free access
Structures		Faculté de médecine / Section de médecine fondamentale / Département de pathologie et immunologie
Research group		Radicaux libres et cellules souches embryonnaires (60)
Citation (ISO format)		BRAULT, Julien et al. Optimized Generation of Functional Neutrophils and Macrophages from Patient-Specific Induced Pluripotent Stem Cells: Ex Vivo Models of X(0)-Linked, AR22(0)- and AR47(0)- Chronic Granulomatous Diseases. In: BioResearch Open Access, 2014, vol. 3, n° 6, p. 311-326. doi: 10.1089/biores.2014.0045 https://archive-ouverte.unige.ch/unige:82438