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Scientific article
English

Automated LC-MS method for the fast stereoselective determination of methadone in plasma

Contributors

Souverain, Sandrine; Eap, Chin; Veuthey, Jean-Luc; Rudaz, Serge

Published in

Clinical chemistry and laboratory medicine, vol. 41, no. 12, p. 1615-1621

Publication date

2003

Abstract

Methadone (MTD) is a chiral drug widely used for the treatment of opioid dependence for which a rapid analytical method for the determination of each enantiomer would be advantageous. In order to improve method sensitivity and to automate the entire analytical process, a column-switching configuration has been developed. An online extraction system coupled to a cellulose-based chiral stationary phase (CSP), namely Chiralcel OJ-R, was used and detection was performed by mass spectrometry. Fifty microl of plasma were injected into the liquid chromatography-mass spectrometry (LC-MS) system after addition of acetonitrile (ACN) containing methadone deuterated D9 (MTD-D9) (internal standard) and centrifugation. For the rapid extraction step, a large particle size support was selected. A baseline separation of MTD enantiomers was obtained in less than 12 min. Trueness and precision were evaluated with control samples at 500 ng/ml of (R,S)-methadone. Trueness values were 106.6% and 103.0% for (R)-MTD and (S)-MTD, respectively, with a coefficient of variation inferior to 2.5% for both compounds. Finally, a good concordance was found using this method for analysis of plasma samples from patients in maintenance treatment as compared to a previously described HPLC-UV method after liquid-liquid extraction.

Keywords

  • Methadone enantiomers
  • Chiralcel OJ-R
  • Plasma samples
  • Liquid chromatography-mass spectrometry: LC-MS
  • Column-switching

UNIGE affiliation entities

Citation (ISO format)

SOUVERAIN, Sandrine et al. Automated LC-MS method for the fast stereoselective determination of methadone in plasma. In: Clinical chemistry and laboratory medicine, 2003, vol. 41, n° 12, p. 1615–1621. doi: 10.1515/CCLM.2003.245

Main files (1)

Article (Published version)

Identifiers

Journal ISSN

1434-6621
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Technical informations

Creation

15/03/2016 15:49:00

First validation

15/03/2016 15:49:00

Update

15/03/2023 00:13:52

Status update

15/03/2023 00:13:51

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