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Scientific article
English

Sphingoid base is required for translation initiation during heat stress in Saccharomyces cerevisiae

Contributors

Meier, Karsten D.; Deloche, Olivier; Kajiwara, Kentaro; Funato, Kouichi; Riezman, Howard

Published in

Molecular biology of the cell, vol. 17, p. 1164-1175

Publication date

2006

Abstract

Sphingolipids are required for many cellular functions including response to heat shock. We analyzed the yeast lcb1-100 mutant, which is conditionally impaired in the first step of sphingolipid biosynthesis and shows a strong decrease in heat shock protein synthesis and viability. Transcription and nuclear export of heat shock protein mRNAs is not affected. However, lcb1-100 cells exhibited a strong decrease in protein synthesis caused by a defect in translation initiation under heat stress conditions. The essential lipid is sphingoid base, not ceramide or sphingoid base phosphates. Deletion of the eIF4E-binding protein Eap1p in lcb-100 cells restored translation of heat shock proteins and increased viability. The translation defect during heat stress in lcb1-100 was due at least partially to a reduced function of the sphingoid base-activated PKH1/2 protein kinases. In addition, depletion of the translation initiation factor eIF4G was observed in lcb1-100 cells and ubiquitin overexpression allowed partial recovery of translation after heat stress. Taken together, we have shown a requirement for sphingoid bases during the recovery from heat shock and suggest that this reflects a direct lipid-dependent signal to the cap-dependent translation initiation apparatus.

UNIGE affiliation entities

Citation (ISO format)

MEIER, Karsten D. et al. Sphingoid base is required for translation initiation during heat stress in Saccharomyces cerevisiae. In: Molecular biology of the cell, 2006, vol. 17, p. 1164–1175.

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Article (Published version)

Identifiers

  • PID : unige:82045

Journal ISSN

1059-1524
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Technical informations

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10/02/2016 11:32:00

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10/02/2016 11:32:00

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15/03/2023 00:13:49

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