en
Scientific article
English

Ultra high performance supercritical fluid chromatography coupled with tandem mass spectrometry for screening of doping agents. II: Analysis of biological samples

Published inAnalytica chimica acta, vol. 853, p. 647-659
Publication date2015
Abstract

The potential and applicability of UHPSFC–MS/MS for anti-doping screening in urine samples were tested for the first time. For this purpose, a group of 110 doping agents with diverse physicochemical properties was analyzed using two separation techniques, namely UHPLC–MS/MS and UHPSFC–MS/MS in both ESI+ and ESI− modes. The two approaches were compared in terms of selectivity, sensitivity, linearity and matrix effects. As expected, very diverse retentions and selectivities were obtained in UHPLC and UHPSFC, proving a good complementarity of these analytical strategies. In both conditions, acceptable peak shapes and MS detection capabilities were obtained within 7 min analysis time, enabling the application of these two methods for screening purposes. Method sensitivity was found comparable for 46% of tested compounds, while higher sensitivity was observed for 21% of tested compounds in UHPLC–MS/MS and for 32% in UHPSFC–MS/MS. The latter demonstrated a lower susceptibility to matrix effects, which were mostly observed as signal suppression. In the case of UHPLC–MS/MS, more serious matrix effects were observed, leading typically to signal enhancement and the matrix effect was also concentration dependent, i.e., more significant matrix effects occurred at the lowest concentrations.

Keywords
  • Ultra high performance supercritical fluid chromatography
  • Ultra high performance liquid chromatography
  • Doping agents
  • Biological samples
  • Urine
  • Matrix effects
Citation (ISO format)
NOVÁKOVÁ, Lucie et al. Ultra high performance supercritical fluid chromatography coupled with tandem mass spectrometry for screening of doping agents. II: Analysis of biological samples. In: Analytica chimica acta, 2015, vol. 853, p. 647–659. doi: 10.1016/j.aca.2014.10.007
Main files (1)
Article (Published version)
accessLevelRestricted
Identifiers
ISSN of the journal0003-2670
509views
3downloads

Technical informations

Creation02/16/2016 4:56:00 PM
First validation02/16/2016 4:56:00 PM
Update time03/15/2023 12:08:43 AM
Status update03/15/2023 12:08:43 AM
Last indexation01/16/2024 8:15:06 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack