Untargeted profiling of urinary steroid metabolites after testosterone ingestion: opening new perspectives for antidoping testing

Boccard, Julien; Badoud, Flavia; Jan, Nicolas; Nicoli, Raul; Schweizer, Carine; Pralong, François; Veuthey, Jean-Luc; Baume, Norbert; Rudaz, Serge; Saugy, Martial

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Title		Untargeted profiling of urinary steroid metabolites after testosterone ingestion: opening new perspectives for antidoping testing
Authors		Boccard, Julien Badoud, Flavia Jan, Nicolas Nicoli, Raul Schweizer, Carine Pralong, François Veuthey, Jean-Luc Baume, Norbert Rudaz, Serge Saugy, Martial show all authors [1 - 10]
Published in		Bioanalysis. 2014, vol. 6, no. 19, p. 2523-2536
Abstract		Antidoping procedures are expected to greatly benefit from untargeted metabolomic approaches through the discovery of new biomarkers of prohibited substances abuse. Results: Endogenous steroid metabolites were monitored in urine samples from a controlled elimination study of testosterone undecanoate after ingestion. A platform coupling ultra-high pressure LC with high-resolution quadrupole TOF MS was used and high between-subject metabolic variability was successfully handled using a multiblock data analysis strategy. Links between specific subsets of metabolites and influential genetic polymorphisms of the UGT2B17 enzyme were highlighted. Conclusion: This exploratory metabolomic strategy constitutes a first step toward a better understanding of the underlying patterns driving the high interindividual variability of steroid metabolism. Promising biomarkers were selected for further targeted study.
Keywords		Doping analysis — Multiblock data analysis — Steroidomics — Testosterone — UHPLC-QTOF/MS — Urine
Identifiers		DOI: 10.4155/bio.14.200
Full text		Article (Published version) (1.2 MB) - Limited access to UNIGE Other version: http://www.future-science.com/doi/abs/10.4155/bio.14.200
Structures		Faculté des sciences / Section des sciences pharmaceutiques
Research group		Sciences analytiques
Citation (ISO format)		BOCCARD, Julien et al. Untargeted profiling of urinary steroid metabolites after testosterone ingestion: opening new perspectives for antidoping testing. In: Bioanalysis, 2014, vol. 6, n° 19, p. 2523-2536. https://archive-ouverte.unige.ch/unige:80746