Development of computational tools to improve data-independent workflows for the characterization of proteins and metabolites by mass spectrometry

Bilbao Pena, Aivett

doi:10.13097/archive-ouverte/unige:80046

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Title		Development of computational tools to improve data-independent workflows for the characterization of proteins and metabolites by mass spectrometry
Author		Bilbao Pena, Aivett
Directors		Hopfgartner, Gerard Lisacek, Frédérique
Defense		Thèse de doctorat : Univ. Genève, 2015 - Sc. 4872 - 2015/12/09
Abstract		This thesis focuses on the design, implementation and benchmarking of various software tools aiming to improve the identification and quantification of complex protein digests and metabolites analyzed by LC-MS/MS. The solutions involve different steps in the workflow, from enhanced data acquisition to novel post-acquisition data processing strategies. A significant increase of peptide/protein identification rates was achieved by combining exclusion and inclusion lists in data-dependent acquisition. Data-independent acquisition schemes are examined, in particular, related algorithms and computational methods are discussed. A program was implemented to design and optimize different SWATH acquisition methods and the benefits of variable isolation windows are demonstrated for the profiling of proteomic and metabolic samples. A ranking algorithm was developed to assign low priority to fragment ions affected by interference during SWATH acquisition and the improvements for label-free quantification are illustrated. Finally, several demultiplexing approaches towards peptide identification by sequence database search of SWATH spectra were investigated.
Keywords		Data-independent acquisition — Interference removal — Metabolomics — Proteomics — SWATH — Variable-precursor-isolation-window widths
Identifiers		DOI: 10.13097/archive-ouverte/unige:80046 URN: urn:nbn:ch:unige-800462
Full text		Thesis (9.8 MB) - Limited access to UNIGE
Structures		Faculté des sciences
Research groups		Spectrométrie de masse du vivant Proteome Informatics Group
Citation (ISO format)		BILBAO PENA, Aivett. Development of computational tools to improve data-independent workflows for the characterization of proteins and metabolites by mass spectrometry. Université de Genève. Thèse, 2015. doi: 10.13097/archive-ouverte/unige:80046 https://archive-ouverte.unige.ch/unige:80046