en
Scientific article
Open access
English

The effect of heterogeneous Transcription Start Sites (TSS) on the translatome: implications for the mammalian cellular phenotype

Published inBMC genomics, vol. 16, no. 1, 986
Publication date2015
Abstract

The genetic program, as manifested as the cellular phenotype, is in large part dictated by the cell's protein composition. Since characterisation of the proteome remains technically laborious it is attractive to define the genetic expression profile using the transcriptome. However, the transcriptional landscape is complex and it is unclear as to what extent it reflects the ribosome associated mRNA population (the translatome). This is particularly pertinent for genes using multiple transcriptional start sites (TSS) generating mRNAs with heterogeneous 5' transcript leaders (5'TL). Furthermore, the relative abundance of the TSS gene variants is frequently cell-type specific. Indeed, promoter switches have been reported in pathologies such as cancer. The consequences of this 5'TL heterogeneity within the transcriptome for the translatome remain unresolved. This is not a moot point because the 5'TL plays a key role in regulating mRNA recruitment onto polysomes.

Funding
  • Swiss National Science Foundation - Ligue Genevoise contre le cancer
Citation (ISO format)
DIEUDONNE, François-Xavier et al. The effect of heterogeneous Transcription Start Sites (TSS) on the translatome: implications for the mammalian cellular phenotype. In: BMC genomics, 2015, vol. 16, n° 1, p. 986. doi: 10.1186/s12864-015-2179-8
Main files (1)
Article (Published version)
accessLevelPublic
Identifiers
ISSN of the journal1471-2164
592views
252downloads

Technical informations

Creation11/23/2015 11:53:00 AM
First validation11/23/2015 11:53:00 AM
Update time03/14/2023 11:57:54 PM
Status update03/14/2023 11:57:53 PM
Last indexation01/16/2024 7:48:43 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack