The effect of heterogeneous Transcription Start Sites (TSS) on the translatome: implications for the mammalian cellular phenotype

Dieudonne, François-Xavier; O’Connor, Patrick B. F.; Jaquier-Gubler, Pascale; Yasrebi, Haleh; Conne, Béatrice; Nikolaev, Sergey Igorievich; Antonarakis, Stylianos; Baranov, Pavel V.; Curran, Joseph

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Title		The effect of heterogeneous Transcription Start Sites (TSS) on the translatome: implications for the mammalian cellular phenotype
Authors		Dieudonne, François-Xavier O’Connor, Patrick B. F. Jaquier-Gubler, Pascale Yasrebi, Haleh Conne, Béatrice Nikolaev, Sergey Igorievich Antonarakis, Stylianos Baranov, Pavel V. Curran, Joseph show all authors [1 - 9]
Published in		BMC Genomics. 2015, vol. 16, no. 1, p. 986
Abstract		The genetic program, as manifested as the cellular phenotype, is in large part dictated by the cell's protein composition. Since characterisation of the proteome remains technically laborious it is attractive to define the genetic expression profile using the transcriptome. However, the transcriptional landscape is complex and it is unclear as to what extent it reflects the ribosome associated mRNA population (the translatome). This is particularly pertinent for genes using multiple transcriptional start sites (TSS) generating mRNAs with heterogeneous 5' transcript leaders (5'TL). Furthermore, the relative abundance of the TSS gene variants is frequently cell-type specific. Indeed, promoter switches have been reported in pathologies such as cancer. The consequences of this 5'TL heterogeneity within the transcriptome for the translatome remain unresolved. This is not a moot point because the 5'TL plays a key role in regulating mRNA recruitment onto polysomes.
Identifiers		DOI: 10.1186/s12864-015-2179-8 PMID: 26589636
Full text		Article (Published version) (3.8 MB) - Free access Other version: http://www.biomedcentral.com/1471-2164/16/986
Structures		Faculté de médecine / Section de médecine fondamentale / Département de médecine génétique et développement Faculté de médecine / Section de médecine fondamentale / Département de microbiologie et médecine moléculaire
Research groups		Régulation traductionnelle dans les cellules de mammifère (631) Pathologie Moléculaire de la Trisomie 21 et le Génome Humain (248)
Project		FNS: Ligue Genevoise contre le cancer
Citation (ISO format)		DIEUDONNE, François-Xavier et al. The effect of heterogeneous Transcription Start Sites (TSS) on the translatome: implications for the mammalian cellular phenotype. In: BMC Genomics, 2015, vol. 16, n° 1, p. 986. https://archive-ouverte.unige.ch/unige:78593