The effect of heterogeneous Transcription Start Sites (TSS) on the translatome: implications for the mammalian cellular phenotype

Article Article scientifique Article The effect of heterogeneous Transcription Start Sites (TSS) on the translatome: implications for the mammalian cellular phenotype eng 2015 author Dieudonne François-Xavier 806183 author O'Connor Patrick B. F. author Jaquier-Gubler Pascale 23175 author Yasrebi Haleh 45084 author Conne Béatrice 117948 author Nikolaev Sergey Igorievich 593779 author Antonarakis Stylianos 22813 author Baranov Pavel V. author Curran Joseph 22465 986 10.1186/s12864-015-2179-8 26589636 1471-2164 http://www.biomedcentral.com/1471-2164/16/986 The genetic program, as manifested as the cellular phenotype, is in large part dictated by the cell's protein composition. Since characterisation of the proteome remains technically laborious it is attractive to define the genetic expression profile using the transcriptome. However, the transcriptional landscape is complex and it is unclear as to what extent it reflects the ribosome associated mRNA population (the translatome). This is particularly pertinent for genes using multiple transcriptional start sites (TSS) generating mRNAs with heterogeneous 5' transcript leaders (5'TL). Furthermore, the relative abundance of the TSS gene variants is frequently cell-type specific. Indeed, promoter switches have been reported in pathologies such as cancer. The consequences of this 5'TL heterogeneity within the transcriptome for the translatome remain unresolved. This is not a moot point because the 5'TL plays a key role in regulating mRNA recruitment onto polysomes. Dewey 576.5 Dewey 616 Swiss National Science Foundation Ligue Genevoise contre le cancer CH 54 Département de microbiologie et médecine moléculaire 21B 135 Département de médecine génétique et développement 21D false 196 Pathologie Moléculaire de la Trisomie 21 et le Génome Humain 248 365 Régulation traductionnelle dans les cellules de mammifère 631 BMC genomics 16 1 66c57384-666c-4384-8158-cb3ae0a261ad Article (Published version) Public Dieudonne_BMC.pdf 4003345 application/pdf openAccess Faculté de médecine / Section de médecine fondamentale / Département de microbiologie et médecine moléculaire Faculté de médecine / Section de médecine fondamentale / Département de médecine génétique et développement

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The effect of heterogeneous Transcription Start Sites (TSS) on the translatome: implications for the mammalian cellular phenotype	BMC genomics			2015	712	358

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Dieudonne, François-Xavier