UNIGE document Scientific Article
previous document  unige:77876  next document
add to browser collection
Title

Coordinate-based co-localization-mediated analysis of arrestin clustering upon stimulation of the C-C chemokine receptor 5 with RANTES/CCL5 analogues

Authors
Malkusch, Sebastian
Heilemann, Mike
Endesfelder, Ulrike
show hidden authors show all authors [1 - 9]
Published in Histochemistry and Cell Biology. 2014, vol. 142, no. 1, p. 69-77
Abstract G protein-coupled receptor activation and desensitization leads to recruitment of arrestin proteins from cytosolic pools to the cell membrane where they form clusters difficult to characterize due to their small size and further mediate receptor internalization. We quantitatively investigated clustering of arrestin 3 induced by potent anti-HIV analogues of the chemokine RANTES after stimulation of the C-C chemokine receptor 5 using single-molecule localization-based super-resolution microscopy. We determined arrestin 3 cluster sizes and relative fractions of arrestin 3 molecules in each cluster through image-based analysis of the localization data by adapting a method originally developed for co-localization analysis from molecular coordinates. We found that only classical agonists in the set of tested ligands were able to efficiently recruit arrestin 3 to clusters mostly larger than 150 nm in size and compare our results with existing data on arrestin 2 clustering induced by the same chemokine analogues.
Keywords AnimalsArrestins/analysis/metabolismCHO CellsCattleCells, CulturedChemokine CCL5/chemistry/pharmacologyCricetulusMicroscopy, ConfocalMicroscopy, FluorescenceProtein Transport/drug effectsReceptors, CCR5/agonists
Identifiers
PMID: 24623038
Full text
Article (Published version) (2 MB) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Research group Hiv (835)
Citation
(ISO format)
TARANCON DIEZ, Laura et al. Coordinate-based co-localization-mediated analysis of arrestin clustering upon stimulation of the C-C chemokine receptor 5 with RANTES/CCL5 analogues. In: Histochemistry and Cell Biology, 2014, vol. 142, n° 1, p. 69-77. https://archive-ouverte.unige.ch/unige:77876

169 hits

0 download

Update

Deposited on : 2015-11-30

Export document
Format :
Citation style :