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Title

Cysteine-string proteins regulate exocytosis of insulin independent from transmembrane ion fluxes

Authors
Zhang, H.
Chamberlain, L. H.
Burgoyne, R. D.
Wollheim, C. B.
Published in FEBS Letters. 1998, vol. 437, no. 3, p. 267-272
Abstract Cysteine-string proteins (Csps) are vesicle proteins involved in exocytosis of synaptic vesicles in Drosophila and modulation of presynaptic calcium influx. As both the contribution of calcium channel regulation to the role of Csp in exocytosis and a function of Csp outside the nervous system are unknown, we studied its function in endocrine exocytosis from large dense core vesicles (LDCVs) using insulin-secreting pancreatic beta-cells. Csps were expressed in primary and derived beta-cell lines on insulin-containing LDCVs. Suppression of Csp expression reduced not only depolarisation induced insulin release but also exocytosis in permeabilised cells directly stimulated by Ca2+. Thus, Csp is a secretory granule protein and is required for endocrine exocytosis independent of the modulation of transmembrane calcium fluxes.
Keywords AnimalsCattleCell Membrane/metabolismCricetinaeDNA, Antisense/biosynthesisExocytosis/drug effectsHSP40 Heat-Shock ProteinsInsulin/ secretionIon Channels/ metabolismMembrane Proteins/biosynthesis/genetics/ physiologyMiceRatsSubcellular Fractions/metabolismTumor Cells, Cultured
Identifiers
PMID: 9824305
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ZHANG, H. et al. Cysteine-string proteins regulate exocytosis of insulin independent from transmembrane ion fluxes. In: FEBS Letters, 1998, vol. 437, n° 3, p. 267-272. https://archive-ouverte.unige.ch/unige:7716

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Deposited on : 2010-06-21

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