Cysteine-string proteins regulate exocytosis of insulin independent from transmembrane ion fluxes

Zhang, H.; Kelley, William; Chamberlain, L. H.; Burgoyne, R. D.; Wollheim, Claes; Lang, Jochen

doi:10.1016/s0014-5793(98)01233-2

Scientific article

English

Cysteine-string proteins regulate exocytosis of insulin independent from transmembrane ion fluxes

Contributors

Zhang, H.; Kelley, William; Chamberlain, L. H.; Burgoyne, R. D.; Wollheim, Claes; Lang, Jochen

Published in

FEBS letters, vol. 437, no. 3, p. 267-272

Publication date

1998

Abstract

Cysteine-string proteins (Csps) are vesicle proteins involved in exocytosis of synaptic vesicles in Drosophila and modulation of presynaptic calcium influx. As both the contribution of calcium channel regulation to the role of Csp in exocytosis and a function of Csp outside the nervous system are unknown, we studied its function in endocrine exocytosis from large dense core vesicles (LDCVs) using insulin-secreting pancreatic beta-cells. Csps were expressed in primary and derived beta-cell lines on insulin-containing LDCVs. Suppression of Csp expression reduced not only depolarisation induced insulin release but also exocytosis in permeabilised cells directly stimulated by Ca2+. Thus, Csp is a secretory granule protein and is required for endocrine exocytosis independent of the modulation of transmembrane calcium fluxes.

Keywords

Animals
Cattle
Cell Membrane/metabolism
Cricetinae
DNA, Antisense/biosynthesis
Exocytosis/drug effects
HSP40 Heat-Shock Proteins
Insulin/ secretion
Ion Channels/ metabolism
Membrane Proteins/biosynthesis/genetics/ physiology
Mice
Rats
Subcellular Fractions/metabolism
Tumor Cells, Cultured

UNIGE affiliation entities

Faculté de médecine / Section de médecine clinique / Département de médecine

Citation (ISO format)

ZHANG, H. et al. Cysteine-string proteins regulate exocytosis of insulin independent from transmembrane ion fluxes. In: FEBS letters, 1998, vol. 437, n° 3, p. 267–272. doi: 10.1016/s0014-5793(98)01233-2

Article

Identifiers

PID : unige:7716
DOI : 10.1016/s0014-5793(98)01233-2
PMID : 9824305

Journal ISSN

0014-5793

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Cysteine-string proteins regulate exocytosis of insulin independent from transmembrane ion fluxes

Contributors

Published in

Publication date

Abstract

Keywords

UNIGE affiliation entities

Citation (ISO format)

Main files (1)

Identifiers

Journal ISSN

Technical informations

Creation

First validation

Update

Status update

Last indexation