Scientific article

Cell-associated HIV-1 RNA in blood as indicator of virus load in lymph nodes. The Swiss HIV Cohort Study

Published inThe Journal of infectious diseases, vol. 180, no. 3, p. 850-853
Publication date1999

We have developed sensitive assays for viremia and cell-associated human immunodeficiency virus type 1 (HIV-1) RNA and DNA to assess the predictive value of virological parameters determined in blood for virus load in lymph nodes (LNs). Eighteen patients were included; 13 received stavudine/didanosine/hydroxyurea and 5 stavudine/didanosine, and all had viremia <500 copies/mL for >3 months. At the time of LN biopsy (median, 10 months), the median viremia was 2.09 log copies/mL (range, <0.70-3.34). Cell-associated HIV-1 RNA and DNA were detectable in blood and LNs of all patients. The median cell-associated RNA and DNA were 2.16 log copies/106 cells and 2.60 log copies/106 cells in blood versus 4.31 log RNA copies/106 cells and 3.26 log DNA copies/106 cells in LNs. Regression analysis shows that, in treated patients with sustained low viremia, cell-associated RNA and DNA in blood are better predictors of virus load in LNs than viremia.

  • Acquired Immunodeficiency Syndrome/ drug therapy/immunology
  • Anti-HIV Agents/ therapeutic use
  • CD4-CD8 Ratio
  • CD4-Positive T-Lymphocytes/immunology/pathology
  • CD8-Positive T-Lymphocytes/immunology/pathology
  • Didanosine/ therapeutic use
  • Drug Therapy, Combination
  • HIV Infections/ drug therapy/immunology
  • HIV-1/ isolation & purification
  • Humans
  • Hydroxyurea/ therapeutic use
  • Lymph Nodes/immunology/pathology/ virology
  • RNA, Viral/ blood
  • Stavudine/ therapeutic use
  • Viral Load
Citation (ISO format)
YERLY FERRILLO, Sabine et al. Cell-associated HIV-1 RNA in blood as indicator of virus load in lymph nodes. The Swiss HIV Cohort Study. In: The Journal of infectious diseases, 1999, vol. 180, n° 3, p. 850–853. doi: 10.1086/314932
Updates (1)
ISSN of the journal0022-1899

Technical informations

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