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Cell-associated HIV-1 RNA in blood as indicator of virus load in lymph nodes. The Swiss HIV Cohort Study

Opravil, M.
Marchal, F.
Published in The Journal of Infectious Diseases. 1999, vol. 180, no. 3, p. 850-853
Abstract We have developed sensitive assays for viremia and cell-associated human immunodeficiency virus type 1 (HIV-1) RNA and DNA to assess the predictive value of virological parameters determined in blood for virus load in lymph nodes (LNs). Eighteen patients were included; 13 received stavudine/didanosine/hydroxyurea and 5 stavudine/didanosine, and all had viremia <500 copies/mL for >3 months. At the time of LN biopsy (median, 10 months), the median viremia was 2.09 log copies/mL (range, <0.70-3.34). Cell-associated HIV-1 RNA and DNA were detectable in blood and LNs of all patients. The median cell-associated RNA and DNA were 2.16 log copies/106 cells and 2.60 log copies/106 cells in blood versus 4.31 log RNA copies/106 cells and 3.26 log DNA copies/106 cells in LNs. Regression analysis shows that, in treated patients with sustained low viremia, cell-associated RNA and DNA in blood are better predictors of virus load in LNs than viremia.
Keywords Acquired Immunodeficiency Syndrome/ drug therapy/immunologyAnti-HIV Agents/ therapeutic useCD4-CD8 RatioCD4-Positive T-Lymphocytes/immunology/pathologyCD8-Positive T-Lymphocytes/immunology/pathologyDidanosine/ therapeutic useDrug Therapy, CombinationHIV Infections/ drug therapy/immunologyHIV-1/ isolation & purificationHumansHydroxyurea/ therapeutic useLymph Nodes/immunology/pathology/ virologyRNA, Viral/ bloodStavudine/ therapeutic useViral Load
PMID: 10438378
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YERLY FERRILLO, Sabine et al. Cell-associated HIV-1 RNA in blood as indicator of virus load in lymph nodes. The Swiss HIV Cohort Study. In: The Journal of Infectious Diseases, 1999, vol. 180, n° 3, p. 850-853. doi: 10.1086/314932 https://archive-ouverte.unige.ch/unige:7707

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Deposited on : 2010-06-21

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