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Title

Decay of cell-associated HIV-1 DNA correlates with residual replication in patients treated during acute HIV-1 infection

Authors
Perneger, T. V.
Vora, S.
Published in AIDS. 2000, vol. 14, no. 18, p. 2805-2812
Abstract OBJECTIVES: To evaluate the decay rate of cell-associated HIV-1 RNA and DNA and to identify factors associated with residual viral load in patients treated at the time of primary HIV-1 infection. PATIENTS: A group of 15 patients adherent to highly active antiretroviral therapy (HAART) with sustained undetectable HIV-1 viremia for at least 24 months. METHODS: Viremia, cell-associated HIV-1 RNA and DNA in blood and lymph node mononuclear cells were measured using ultrasensitive assays. RESULTS: Viremia decreased rapidly in all patients; HIV RNA remained < 3 copies/ml in nine patients and fluctuated between 3 and 50 copies/ml in five patients and between 50 and 200 copies/ml in one patient. Decay rates of cell-associated RNA and DNA presented an inflexion point at 1 and 3 months, respectively: first-phase mean half-lives were 0.15 and 0.84 months, respectively, and second-phase mean half-lives were 13.7 and 6.6 months, respectively (95% confidence interval 4.4-13.8). The second phase decay rates were markedly slower, with a DNA decay rate that was highly associated with the mean levels of cell-associated RNA measured in blood from 6 to 33 months (P= 0.001) and in lymph nodes collected at 14 months (P= 0.02). CONCLUSIONS: The clearance of HIV-1 infected cells is correlated with the extent of viral replication as measured by cell-associated RNA levels in both blood and lymph nodes. Quantification of cell-associated RNA and DNA further defines treatment efficacy in 'aviremic' patients.
Keywords AdultAntiretroviral Therapy, Highly ActiveDNA, Viral/analysis/ bloodFemaleHIV Infections/ drug therapy/virologyHIV-1/ physiologyHumansLeukocytes, Mononuclear/virologyLymph Nodes/virologyMaleMiddle AgedRNA, Viral/analysis/bloodViral LoadViremia/virologyVirus Replication
Identifiers
PMID: 11153661
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YERLY FERRILLO, Sabine et al. Decay of cell-associated HIV-1 DNA correlates with residual replication in patients treated during acute HIV-1 infection. In: AIDS, 2000, vol. 14, n° 18, p. 2805-2812. https://archive-ouverte.unige.ch/unige:7704

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Deposited on : 2010-06-21

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