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Scientific article
English

Decay of cell-associated HIV-1 DNA correlates with residual replication in patients treated during acute HIV-1 infection

Contributors

Yerly Ferrillo, Sabine; Perneger, T. V.; Vora, S.; Hirschel, Bernard; Perrin, Luc

Published in

AIDS, vol. 14, no. 18, p. 2805-2812

Publication date

2000

Abstract

OBJECTIVES: To evaluate the decay rate of cell-associated HIV-1 RNA and DNA and to identify factors associated with residual viral load in patients treated at the time of primary HIV-1 infection. PATIENTS: A group of 15 patients adherent to highly active antiretroviral therapy (HAART) with sustained undetectable HIV-1 viremia for at least 24 months. METHODS: Viremia, cell-associated HIV-1 RNA and DNA in blood and lymph node mononuclear cells were measured using ultrasensitive assays. RESULTS: Viremia decreased rapidly in all patients; HIV RNA remained < 3 copies/ml in nine patients and fluctuated between 3 and 50 copies/ml in five patients and between 50 and 200 copies/ml in one patient. Decay rates of cell-associated RNA and DNA presented an inflexion point at 1 and 3 months, respectively: first-phase mean half-lives were 0.15 and 0.84 months, respectively, and second-phase mean half-lives were 13.7 and 6.6 months, respectively (95% confidence interval 4.4-13.8). The second phase decay rates were markedly slower, with a DNA decay rate that was highly associated with the mean levels of cell-associated RNA measured in blood from 6 to 33 months (P= 0.001) and in lymph nodes collected at 14 months (P= 0.02). CONCLUSIONS: The clearance of HIV-1 infected cells is correlated with the extent of viral replication as measured by cell-associated RNA levels in both blood and lymph nodes. Quantification of cell-associated RNA and DNA further defines treatment efficacy in 'aviremic' patients.

Keywords

  • Adult
  • Antiretroviral Therapy, Highly Active
  • DNA, Viral/analysis/ blood
  • Female
  • HIV Infections/ drug therapy/virology
  • HIV-1/ physiology
  • Humans
  • Leukocytes, Mononuclear/virology
  • Lymph Nodes/virology
  • Male
  • Middle Aged
  • RNA, Viral/analysis/blood
  • Viral Load
  • Viremia/virology
  • Virus Replication

UNIGE affiliation entities

Citation (ISO format)

YERLY FERRILLO, Sabine et al. Decay of cell-associated HIV-1 DNA correlates with residual replication in patients treated during acute HIV-1 infection. In: AIDS, 2000, vol. 14, n° 18, p. 2805–2812.

Main files (1)

Article

Identifiers

Additional URL for this publication

http://journals.lww.com/aidsonline/pages/articleviewer.aspx?year=2000&issue=12220&article=00001&type=abstract

Journal ISSN

0269-9370
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Technical informations

Creation

21/06/2010 10:27:13

First validation

21/06/2010 10:27:13

Update

14/03/2023 15:46:17

Status update

14/03/2023 15:46:17

Last indexation

29/10/2024 15:22:06
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