UNIGE document Scientific Article
previous document  unige:76657  next document
add to browser collection

WASH is required for lysosomal recycling and efficient autophagic and phagocytic digestion

King, Jason S
Insall, Robert H
Published in Molecular Biology of the Cell. 2013, vol. 24, no. 17, p. 2714-2726
Abstract Wiskott-Aldrich syndrome protein and SCAR homologue (WASH) is an important regulator of vesicle trafficking. By generating actin on the surface of intracellular vesicles, WASH is able to directly regulate endosomal sorting and maturation. We report that, in Dictyostelium, WASH is also required for the lysosomal digestion of both phagocytic and autophagic cargo. Consequently, Dictyostelium cells lacking WASH are unable to grow on many bacteria or to digest their own cytoplasm to survive starvation. WASH is required for efficient phagosomal proteolysis, and proteomic analysis demonstrates that this is due to reduced delivery of lysosomal hydrolases. Both protease and lipase delivery are disrupted, and lipid catabolism is also perturbed. Starvation-induced autophagy therefore leads to phospholipid accumulation within WASH-null lysosomes. This causes the formation of multilamellar bodies typical of many lysosomal storage diseases. Mechanistically, we show that, in cells lacking WASH, cathepsin D becomes trapped in a late endosomal compartment, unable to be recycled to nascent phagosomes and autophagosomes. WASH is therefore required for the maturation of lysosomes to a stage at which hydrolases can be retrieved and reused.
Keywords Actin-Related Protein 2-3 Complex/metabolismActins/metabolismAutophagyCathepsin D/metabolismDictyostelium/metabolismEndosomes/metabolismLysosomes/metabolismMicrofilament Proteins/genetics/metabolismPhagocytosisPhagosomes/physiologyProtein TransportProteomicsProtozoan Proteins/genetics/metabolismVesicular Transport Proteins/genetics/metabolismBeta-N-Acetylhexosaminidases/metabolism
PMID: 23885127
Full text
Article (Published version) (2.5 MB) - public document Free access
(ISO format)
KING, Jason S et al. WASH is required for lysosomal recycling and efficient autophagic and phagocytic digestion. In: Molecular Biology of the Cell, 2013, vol. 24, n° 17, p. 2714-2726. doi: 10.1091/mbc.E13-02-0092 https://archive-ouverte.unige.ch/unige:76657

458 hits



Deposited on : 2015-10-28

Export document
Format :
Citation style :