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16S rRNA sequence diversity in Mycobacterium celatum strains caused by presence of two different copies of 16S rRNA gene

Publié dansJournal of clinical microbiology, vol. 36, no. 6, p. 1761-1764
Date de publication1998
Résumé

Direct sequencing of the 16S rRNA gene (16S rDNA) of Mycobacterium celatum isolates showed ambiguities, suggesting heterogeneity. Cloned 16S rDNA yielded two copies of the gene, which differed by insertion of a thymine at position 214 and by additional mismatches. Restriction fragment length polymorphism analysis confirmed the presence of two copies of 16S rDNA within the bacterial chromosome.

Mots-clés
  • Base Sequence
  • Cloning, Molecular
  • DNA, Bacterial/ analysis
  • DNA, Ribosomal/ analysis
  • Genes, Bacterial
  • Genetic Variation
  • Humans
  • Molecular Sequence Data
  • Mycobacterium/classification/ genetics/isolation & purification
  • Polymorphism, Restriction Fragment Length
  • RNA, Ribosomal, 16S/ genetics
  • Sequence Alignment
  • RRNA Operon
Citation (format ISO)
REISCHL, U. et al. 16S rRNA sequence diversity in Mycobacterium celatum strains caused by presence of two different copies of 16S rRNA gene. In: Journal of clinical microbiology, 1998, vol. 36, n° 6, p. 1761–1764.
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Article
accessLevelRestricted
Identifiants
ISSN du journal0095-1137
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Création21/06/2010 10:26:09
Première validation21/06/2010 10:26:09
Heure de mise à jour14/03/2023 15:45:20
Changement de statut14/03/2023 15:45:20
Dernière indexation12/02/2024 18:36:35
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