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Scientific article
English

16S rRNA sequence diversity in Mycobacterium celatum strains caused by presence of two different copies of 16S rRNA gene

ContributorsReischl, U.; Feldmann, K.; Naumann, L.; Gaugler, B. J.; Bescher Ninet, Béatrice Alice; Hirschel, Bernard; Emler, Stefan
Published inJournal of clinical microbiology, vol. 36, no. 6, p. 1761-1764
Publication date1998
Abstract

Direct sequencing of the 16S rRNA gene (16S rDNA) of Mycobacterium celatum isolates showed ambiguities, suggesting heterogeneity. Cloned 16S rDNA yielded two copies of the gene, which differed by insertion of a thymine at position 214 and by additional mismatches. Restriction fragment length polymorphism analysis confirmed the presence of two copies of 16S rDNA within the bacterial chromosome.

Keywords
  • Base Sequence
  • Cloning, Molecular
  • DNA, Bacterial/ analysis
  • DNA, Ribosomal/ analysis
  • Genes, Bacterial
  • Genetic Variation
  • Humans
  • Molecular Sequence Data
  • Mycobacterium/classification/ genetics/isolation & purification
  • Polymorphism, Restriction Fragment Length
  • RNA, Ribosomal, 16S/ genetics
  • Sequence Alignment
  • RRNA Operon
Affiliation entities
Citation (ISO format)
REISCHL, U. et al. 16S rRNA sequence diversity in Mycobacterium celatum strains caused by presence of two different copies of 16S rRNA gene. In: Journal of clinical microbiology, 1998, vol. 36, n° 6, p. 1761–1764.
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Additional URL for this publicationhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC104915/pdf/jm001761.pdf
Journal ISSN0095-1137
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