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Microaerophilic conditions promote growth of Mycobacterium genavense

De Ridder, K.
Palomino, J.
Portaels, F.
Published in Journal of clinical microbiology. 1998, vol. 36, no. 9, p. 2565-2570
Abstract Our studies show that microaerophilic conditions promote the growth of Mycobacterium genavense in semisolid medium. The growth of M. genavense at 2.5 or 5% oxygen was superior to that obtained at 21% oxygen in BACTEC primary cultures (Middlebrook 7H12, pH 6.0, without additives). By using nondecontaminated specimens, it was possible to detect growth with very small inocula (25 bacilli/ml) of 12 different M. genavense strains (from nude mice) within 6 weeks of incubation under low oxygen tension; conversely, with 21% oxygen, no growth of 8 of 12 (66.7%) M. genavense strains was detected (growth index, <10). The same beneficial effect of 2.5 or 5% oxygen was observed in primary cultures of a decontaminated clinical specimen. Low oxygen tension (2.5 or 5%) is recommended for the primary isolation of M. genavense. Microaerophilic cultivation of other atypical mycobacteria, especially slow-growing (e.g., Mycobacterium avium) and difficult-to-grow (e.g., Mycobacterium ulcerans) species, is discussed.
Keywords AIDS-Related Opportunistic Infections/microbiologyAerobiosisAnimalsCulture MediaHumansMiceMice, NudeMycobacterium/ growth & development/isolation & purificationMycobacterium Infections/complications/microbiologyOxygenTime Factors
PMID: 9705393
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REALINI, Laurence et al. Microaerophilic conditions promote growth of Mycobacterium genavense. In: Journal of clinical microbiology, 1998, vol. 36, n° 9, p. 2565-2570. https://archive-ouverte.unige.ch/unige:7537

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Deposited on : 2010-06-21

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