en
Scientific article
English

Microaerophilic conditions promote growth of Mycobacterium genavense

Published inJournal of clinical microbiology, vol. 36, no. 9, p. 2565-2570
Publication date1998
Abstract

Our studies show that microaerophilic conditions promote the growth of Mycobacterium genavense in semisolid medium. The growth of M. genavense at 2.5 or 5% oxygen was superior to that obtained at 21% oxygen in BACTEC primary cultures (Middlebrook 7H12, pH 6.0, without additives). By using nondecontaminated specimens, it was possible to detect growth with very small inocula (25 bacilli/ml) of 12 different M. genavense strains (from nude mice) within 6 weeks of incubation under low oxygen tension; conversely, with 21% oxygen, no growth of 8 of 12 (66.7%) M. genavense strains was detected (growth index, <10). The same beneficial effect of 2.5 or 5% oxygen was observed in primary cultures of a decontaminated clinical specimen. Low oxygen tension (2.5 or 5%) is recommended for the primary isolation of M. genavense. Microaerophilic cultivation of other atypical mycobacteria, especially slow-growing (e.g., Mycobacterium avium) and difficult-to-grow (e.g., Mycobacterium ulcerans) species, is discussed.

Keywords
  • AIDS-Related Opportunistic Infections/microbiology
  • Aerobiosis
  • Animals
  • Culture Media
  • Humans
  • Mice
  • Mice, Nude
  • Mycobacterium/ growth & development/isolation & purification
  • Mycobacterium Infections/complications/microbiology
  • Oxygen
  • Time Factors
Citation (ISO format)
REALINI, Laurence et al. Microaerophilic conditions promote growth of Mycobacterium genavense. In: Journal of clinical microbiology, 1998, vol. 36, n° 9, p. 2565–2570.
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ISSN of the journal0095-1137
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