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Scientific Article
unige:7537 
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Microaerophilic conditions promote growth of Mycobacterium genavense |
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| Authors | ||
| Published in | Journal of Clinical Microbiology. 1998, vol. 36, no. 9, p. 2565-2570 | |
| Abstract | Our studies show that microaerophilic conditions promote the growth of Mycobacterium genavense in semisolid medium. The growth of M. genavense at 2.5 or 5% oxygen was superior to that obtained at 21% oxygen in BACTEC primary cultures (Middlebrook 7H12, pH 6.0, without additives). By using nondecontaminated specimens, it was possible to detect growth with very small inocula (25 bacilli/ml) of 12 different M. genavense strains (from nude mice) within 6 weeks of incubation under low oxygen tension; conversely, with 21% oxygen, no growth of 8 of 12 (66.7%) M. genavense strains was detected (growth index, <10). The same beneficial effect of 2.5 or 5% oxygen was observed in primary cultures of a decontaminated clinical specimen. Low oxygen tension (2.5 or 5%) is recommended for the primary isolation of M. genavense. Microaerophilic cultivation of other atypical mycobacteria, especially slow-growing (e.g., Mycobacterium avium) and difficult-to-grow (e.g., Mycobacterium ulcerans) species, is discussed. | |
| Keywords | AIDS-Related Opportunistic Infections/microbiology — Aerobiosis — Animals — Culture Media — Humans — Mice — Mice, Nude — Mycobacterium/ growth & development/isolation & purification — Mycobacterium Infections/complications/microbiology — Oxygen — Time Factors | |
| Identifiers | PMID: 9705393 | |
| Full text |
Article - Limited access to UNIGE Other version: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC105163/pdf/jm002565.pdf |
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| Citation (ISO format) | REALINI, Laurence et al. Microaerophilic conditions promote growth of Mycobacterium genavense. In: Journal of Clinical Microbiology, 1998, vol. 36, n° 9, p. 2565-2570. https://archive-ouverte.unige.ch/unige:7537 |
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