UNIGE document Scientific Article
previous document  unige:75317  next document
add to browser collection
Title

Ectopic gene expression and organogenesis in Arabidopsis mutants missing BRU1 required for genome maintenance

Authors
Ohno, Yusuke
Narangajavana, Jarunya
Yamamoto, Akiko
Hattori, Tsukaho
Kagaya, Yasuaki
Gruissem, Wilhelm
Hennig, Lars
show hidden authors show all authors [1 - 9]
Published in Genetics. 2011, vol. 189, no. 1, p. 83-95
Abstract Chromatin reconstitution after DNA replication and repair is essential for the inheritance of epigenetic information, but mechanisms underlying such a process are still poorly understood. Previously, we proposed that Arabidopsis BRU1 functions to ensure the chromatin reconstitution. Loss-of-function mutants of BRU1 are hypersensitive to genotoxic stresses and cause release of transcriptional gene silencing of heterochromatic genes. In this study, we show that BRU1 also plays roles in gene regulation in euchromatic regions. bru1 mutations caused sporadic ectopic expression of genes, including those that encode master regulators of developmental programs such as stem cell maintenance and embryogenesis. bru1 mutants exhibited adventitious organogenesis, probably due to the misexpression of such developmental regulators. The key regulatory genes misregulated in bru1 alleles were often targets of PcG SET-domain proteins, although the overlap between the bru1-misregulated and PcG SET-domain-regulated genes was limited at a genome-wide level. Surprisingly, a considerable fraction of the genes activated in bru1 were located in several subchromosomal regions ranging from 174 to 944 kb in size. Our results suggest that BRU1 has a function related to the stability of subchromosomal gene regulation in the euchromatic regions, in addition to the maintenance of chromatin states coupled with heritable epigenetic marks.
Keywords Arabidopsis/embryology/genetics/metabolismArabidopsis Proteins/genetics/metabolismCell Nucleus/metabolismChromosome MappingChromosomesPlantGene Expression ProfilingGene Expression RegulationDevelopmentalGene Expression RegulationPlantGenomePlantMutation/geneticsOrganogenesis/geneticsPolycomb-Group ProteinsRepressor Proteins/metabolismTranscription Factors/genetics
Identifiers
PMID: 21705754
Full text
Article (Published version) (4.2 MB) - document accessible for UNIGE members only Limited access to UNIGE
Other version: http://www.genetics.org/content/189/1/83.long
Structures
Citation
(ISO format)
OHNO, Yusuke et al. Ectopic gene expression and organogenesis in Arabidopsis mutants missing BRU1 required for genome maintenance. In: Genetics, 2011, vol. 189, n° 1, p. 83-95. https://archive-ouverte.unige.ch/unige:75317

157 hits

0 download

Update

Deposited on : 2015-09-22

Export document
Format :
Citation style :