Ectopic gene expression and organogenesis in Arabidopsis mutants missing BRU1 required for genome maintenance

Article Article scientifique Article Ectopic gene expression and organogenesis in Arabidopsis mutants missing BRU1 required for genome maintenance eng 2011 author Ohno Yusuke author Narangajavana Jarunya author Yamamoto Akiko author Hattori Tsukaho author Kagaya Yasuaki author Paszkowski Jerzy 134849 author Gruissem Wilhelm author Hennig Lars author Takeda Shin 566183 83-95 10.1534/genetics.111.130062 21705754 0016-6731 http://www.genetics.org/content/189/1/83.long Chromatin reconstitution after DNA replication and repair is essential for the inheritance of epigenetic information, but mechanisms underlying such a process are still poorly understood. Previously, we proposed that Arabidopsis BRU1 functions to ensure the chromatin reconstitution. Loss-of-function mutants of BRU1 are hypersensitive to genotoxic stresses and cause release of transcriptional gene silencing of heterochromatic genes. In this study, we show that BRU1 also plays roles in gene regulation in euchromatic regions. bru1 mutations caused sporadic ectopic expression of genes, including those that encode master regulators of developmental programs such as stem cell maintenance and embryogenesis. bru1 mutants exhibited adventitious organogenesis, probably due to the misexpression of such developmental regulators. The key regulatory genes misregulated in bru1 alleles were often targets of PcG SET-domain proteins, although the overlap between the bru1-misregulated and PcG SET-domain-regulated genes was limited at a genome-wide level. Surprisingly, a considerable fraction of the genes activated in bru1 were located in several subchromosomal regions ranging from 174 to 944 kb in size. Our results suggest that BRU1 has a function related to the stability of subchromosomal gene regulation in the euchromatic regions, in addition to the maintenance of chromatin states coupled with heritable epigenetic marks. Arabidopsis/embryology/genetics/metabolism Arabidopsis Proteins/genetics/metabolism Cell Nucleus/metabolism Chromosome Mapping Chromosomes Plant Gene Expression Profiling Gene Expression Regulation Developmental Gene Expression Regulation Plant Genome Plant Mutation/genetics Organogenesis/genetics Polycomb-Group Proteins Repressor Proteins/metabolism Transcription Factors/genetics Dewey 580 45 Département des sciences végétales 163 false Genetics 189 1 f0705777-d730-48ef-ad23-4947a20a4a28 Article (Published version) Restricted Ohno_Genetics_2011.pdf 4452605 application/pdf restrictedAccess Faculté des sciences / Section de biologie / Département des sciences végétales

Title	Published in	Access level	OA Policy	Year	Views	Downloads

Ectopic gene expression and organogenesis in Arabidopsis mutants missing BRU1 required for genome maintenance	Genetics			2011	514	0

Archive ouverte UNIGE

Takeda, Shin