en
Doctoral thesis
English

Acute oxidtive stress responses in the microalga Chlamydomonas reinhardtii exposed to metal oxide nanoparticles

Defense date2015-06-29
Abstract

This thesis treats the subject of the potential impact of engineered metal oxide nanomaterials (ENMs) on environmental health and covers aspects from the exposure side to the associated biological hazards. The main objective was to shed light on the short-term dynamics of potential sub-toxic, ENM induced pro-oxidant processes, to develop the link between cell-free and cellular prooxidant processes and to unravel modifying factors thereof. Chlamydomonas reinhardtii served as a model organism and was exposed to nano-CuO and nano-TiO2. Major findings include the observation that agglomeration does not preclude ENM induced pro-oxidant effects, that ambient exposure conditions play a decisive role in determining the toxicity-mediating factor (i.e. particles vs. ions vs mobile ROS) and significantly influence the short-term dynamics of cellular oxidative stress responses and finally, the observation that extra- and intracellular pro-oxidant processes can differ significantly, which may give rise to misleading and conflicting conclusions when considered individually.

eng
Keywords
  • Nanoecotoxicology
  • Risk assessment
  • Reactive oxygen species
  • Pro-oxidant effects
  • Flow cytometry
Funding
  • Swiss National Science Foundation - NRP 64
Citation (ISO format)
VON MOOS, Nadia Rachel. Acute oxidtive stress responses in the microalga Chlamydomonas reinhardtii exposed to metal oxide nanoparticles. 2015. doi: 10.13097/archive-ouverte/unige:75274
Main files (1)
Thesis
accessLevelRestricted
Identifiers
751views
40downloads

Technical informations

Creation09/11/2015 1:22:00 PM
First validation09/11/2015 1:22:00 PM
Update time03/14/2023 11:37:21 PM
Status update03/14/2023 11:37:21 PM
Last indexation05/02/2024 4:25:14 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack