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Scientific article
English

Is cytosolic ionized calcium regulating neutrophil activation?

Published inScience, vol. 221, no. 4618, p. 1413-1415
Publication date1983
Abstract

The concentration of cytosolic ionized calcium, [Ca2+]i, was measured in intact neutrophils by use of a fluorescent indicator trapped in the icytoplasm. A given rise of [Ca2+]i elicited by the chemotactic peptide formylmethionylleucylphenylalanine (FMLP) was associated with a much greater degree of superoxide generation and myeloperoxidase secretion than was the same or larger [Ca2+]i produced by a specific calcium ionophore, ionomycin, which bypasses cell surface receptors. Thus, FMLP appears to generate some important excitatory signal in addition to a rise in [Ca2+]i and exocytosis and superoxide generation in neutrophils may not be simply dependent on [Ca2+]i as is widely supposed.

Keywords
  • Calcium/ physiology
  • Cytoplasm/physiology
  • Ethers/pharmacology
  • Exocytosis
  • Humans
  • Ionomycin
  • Ionophores/pharmacology
  • Lysosomes/enzymology
  • N-Formylmethionine/analogs & derivatives/pharmacology
  • N-Formylmethionine Leucyl-Phenylalanine
  • Neutrophils/ physiology
  • Oligopeptides/pharmacology
  • Oxygen/ metabolism
  • Superoxides/ metabolism
Citation (ISO format)
POZZAN, T. et al. Is cytosolic ionized calcium regulating neutrophil activation? In: Science, 1983, vol. 221, n° 4618, p. 1413–1415. doi: 10.1126/science.6310757
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ISSN of the journal0036-8075
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