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Scientific article
English

Is cytosolic ionized calcium regulating neutrophil activation?

Contributors

Pozzan, T.; Lew, Daniel Pablo; Wollheim, Claes; Tsien, R. Y.

Published in

Science, vol. 221, no. 4618, p. 1413-1415

Publication date

1983

Abstract

The concentration of cytosolic ionized calcium, [Ca2+]i, was measured in intact neutrophils by use of a fluorescent indicator trapped in the icytoplasm. A given rise of [Ca2+]i elicited by the chemotactic peptide formylmethionylleucylphenylalanine (FMLP) was associated with a much greater degree of superoxide generation and myeloperoxidase secretion than was the same or larger [Ca2+]i produced by a specific calcium ionophore, ionomycin, which bypasses cell surface receptors. Thus, FMLP appears to generate some important excitatory signal in addition to a rise in [Ca2+]i and exocytosis and superoxide generation in neutrophils may not be simply dependent on [Ca2+]i as is widely supposed.

Keywords

  • Calcium/ physiology
  • Cytoplasm/physiology
  • Ethers/pharmacology
  • Exocytosis
  • Humans
  • Ionomycin
  • Ionophores/pharmacology
  • Lysosomes/enzymology
  • N-Formylmethionine/analogs & derivatives/pharmacology
  • N-Formylmethionine Leucyl-Phenylalanine
  • Neutrophils/ physiology
  • Oligopeptides/pharmacology
  • Oxygen/ metabolism
  • Superoxides/ metabolism

UNIGE affiliation entities

Citation (ISO format)

POZZAN, T. et al. Is cytosolic ionized calcium regulating neutrophil activation? In: Science, 1983, vol. 221, n° 4618, p. 1413–1415. doi: 10.1126/science.6310757

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Article

Identifiers

Journal ISSN

0036-8075
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