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Scientific article
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Immunocytochemical study on the intracellular localization of the type 2 glucocorticoid receptor in the rat brain

Published inBrain research, vol. 436, no. 1, p. 120-128
Publication date1987
Abstract

The localization of the glucocorticoid receptor (GR) (type 2) in the rat brain was studied with immunocytochemistry using a monoclonal antibody against the rat liver GR. Strong GR immunoreactivity (GR-ir) was observed in neurons of limbic and brainstem structures known to be associated with the stress-activated circuitry, which suggest that these sites are responsive to glucocorticoid feedback. The intracellular localization of GR-ir was examined in CA1 and CA2 pyramidal neurons of the hippocampus. In intact rats GR-ir is predominantly present in the cell nucleus. Adrenalectomy (ADX) caused a slow depletion of the GR-ir signal from the cell nucleus until near detection limits at two weeks postsurgery. At that time, 1 h after administration to longterm ADX rats the synthetic glucocorticoid (type 2) agonist RU 28362 as well as a moderate and high dose of corticosterone (CORT) markedly enhanced the cell nuclear GR-ir. The type 2 antagonist RU 38486 also caused an increase of GR immunostaining in cell nuclei upon acute administration to ADX rats. The mineralocorticoid aldosterone (ALDO) and a low dose of CORT, which bind almost exclusively to type 1 corticosteroid receptors, were ineffective. In conclusion, our data suggest that in the hippocampal CA1-2 neurons type 1 and type 2 corticosteroid receptors may coexist. The steroid-induced changes in cell nuclear immunoreactive GR staining intensity suggest possible cytoplasmic-cell nuclear translocation of GR and/or exposure of immunogenic GR domains.

Keywords
  • Animals
  • Brain Chemistry
  • Brain Stem/analysis
  • Hippocampus/analysis
  • Immunologic Techniques
  • Male
  • Rats
  • Rats, Inbred Strains
  • Receptors, Glucocorticoid/analysis
Citation (ISO format)
VAN EEKELEN, J A et al. Immunocytochemical study on the intracellular localization of the type 2 glucocorticoid receptor in the rat brain. In: Brain research, 1987, vol. 436, n° 1, p. 120–128. doi: 10.1016/0006-8993(87)91563-0
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ISSN of the journal0006-8993
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